Abcam, ab191606, Anti-PI 3 Kinase p85 alpha antibody [EPR18702]

Size: 20µL / 100µL / 1mL
Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (ab191606) is a rabbit monoclonal antibody detecting PI 3 Kinase p85 alpha in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, ICC/IF . Suitable for African green monkey, Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 220 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR18702,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human, African green monkey,
Applications:Flow Cyt (Intra), WB, ICC/IF, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (ab191606) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in African green monkey, Human, Mouse, Rat samples.
What is the molecular weight of PI 3 Kinase p85 alpha?
Anti-PI 3 Kinase p85 alpha [EPR18702] (ab191606) specifically detects a band for PI 3 Kinase p85 alpha (UniProt: Q63787) at a molecular weight of 84kDa.
Trusted by the scientific community
Anti-PI 3 Kinase p85 alpha [EPR18702] (ab191606) was first used in a scientific publication in 2016 and has been cited over 220 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
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Specificity confirmed
The specificity of Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (ab191606) has been confirmed by Western blot testing in PIK3R1 Knockout HAP1 cells.
Other related products
We have a range of other formats of antibody clone [EPR18702] also available for your convenience: ab191606, Carrier free -
ab223792
, APC -
ab225369
, PE -
ab225370
, Alexa Fluor® 488 -
ab225371
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PI 3 Kinase p85 alpha also known as PI3KR1 or phospho PI3K is a regulatory subunit of phosphoinositide 3-kinase (PI3K) that plays a fundamental role in various signaling pathways. The p85 protein has a molecular weight of approximately 85 kDa. This protein is widely expressed in many cell types facilitating essential cellular functions by forming a heterodimer with the class I PI3K catalytic subunit often implicated in regulating lipid signaling pathways.
Biological function summary
P85 alpha modulates intracellular signaling by interacting with other proteins in a complex formation with catalytic subunit p110. This interaction enables the initiation of downstream signaling cascades controlling cell growth proliferation and survival. PI 3 kinase activity mediated by p85 alpha is integral to cellular homeostasis and metabolism helping to execute cellular responses to a variety of growth factors and hormones.
Pathways
PI 3 Kinase p85 alpha integrates into two principal signaling networks: the PI3K-Akt and mTOR pathways. These pathways are instrumental for regulating anabolic processes and cell survival. The p85 form of PI3K interacts with additional proteins such as Akt and mTOR coordinating cellular energy balance promoting protein synthesis and inhibiting apoptosis. Through these interactions the protein ensures that cells respond accurately to nutritional and stress signals.
Alterations in PI 3 Kinase p85 alpha function have been linked to cancer and type 2 diabetes. Aberrant signaling involving PI3K-Akt-mTOR pathways due to mutations in PI3KR1 can contribute to tumor progression and chemoresistance. Moreover dysfunction of this pathway affects insulin signaling connecting p85 alpha to diabetic pathologies. Proteins such as IRS-1 (insulin receptor substrate 1) act alongside p85 alpha in mediating these disease-associated processes emphasizing its importance in maintaining physiological balance.