Abcam, ab192147, Human Follistatin ELISA Kit

Size: 1 x 96Tests
Human Follistatin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Follistatin with a sensitivity of 28 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 28 pg/mL,
Range:0.08 - 5 ng/mL,
Assay time:1h 30m,
Assay Platform:Microplate

Product details:
Human Follistatin ELISA Kit ab192147 is a rapid single-wash 90-min Sandwich ELISA to measure Human Follistatin in cell culture supernatant, EDTA plasma, serum. This SimpleStep sensitivity is 28 pg/mL.
How the assay works
Human Follistatin SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Follistatin ELISA Kit ab192147 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Follistatin is a single-chain polypeptide consisting of about 344 amino acids. The follistatin protein is encoded by a relatively small gene size of 6 kb and 6 exons with alternative splice variants and post translational modifications to give a variety of molecular weights between 31 – 39 kDa. Follistatin forms a high affinity complex with activin sequestering it from its receptor thus having an inhibitory effect. Follistatin acts to promote muscle growth in mammals by acting as an antagonist to the protein myostatin. Myostatin acts as an inhibitor to muscle growth.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Follistatin also known as FS or FST is a glycoprotein with an approximate mass of 32 kDa. This protein is part of the follistatin family and plays an important role in various physiological mechanisms. Follistatin synthesizes and secretes in different tissues including the liver gonads and skeletal muscles. It binds to and inhibits members of the transforming growth factor-beta (TGF-?) superfamily particularly activin. This binding prevents activin from interacting with its receptors.
Biological function summary
Follistatin modulates multiple processes such as cell growth differentiation and reproductive physiology. It acts as a binding protein for activin neutralizing its activity. By preventing activin from engaging with its receptor complexes follistatin influences developmental and regenerative processes. Despite not being a part of a transmembrane complex its interaction with activin impacts extracellular interactions.
Pathways
Follistatin plays a significant role in the regulation of the TGF-? signaling pathway and the muscle growth pathway. In the TGF-? pathway it counteracts the signaling of activin which influences cellular homeostasis and inflammatory responses. In the muscle growth pathway follistatin interferes with myostatin another member of the TGF-? family promoting muscle growth. This cross-talk with proteins such as myostatin demonstrates follistatin's influence in muscle regeneration and development.
Follistatin has clear associations with muscle-related and reproductive disorders. It is linked to muscle wasting diseases such as muscular dystrophy due to its inhibitory role on myostatin known to restrain muscle growth. Follistatin abnormalities can also contribute to conditions like polycystic ovary syndrome (PCOS) due to its regulation of reproductive processes. These ties display follistatin's vital interactions with proteins like activin and myostatin highlighting its importance in disease pathways.