Abcam, ab192306, Anti-MT-ND3 antibody

Size: 100µg
Rabbit Polyclonal MT-ND3 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human MT-ND3 aa 1-100.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MT-ND3 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.P03897

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab192306 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)., Storage buffer-pH: 7.2Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MT-ND3 also known as NADH-ubiquinone oxidoreductase chain 3 is a subunit of Complex I of the mitochondrial respiratory chain. It operates as part of the first enzyme in the electron transport chain which is important for ATP production. With an approximate molecular mass of 12 kDa MT-ND3 is expressed in the inner mitochondrial membrane. This subunit plays a critical role in the transfer of electrons from NADH to ubiquinone facilitating energy conversion within the cell.
Biological function summary
MT-ND3 contributes to essential energy metabolism processes by being integrated into Complex I also known as NADH: ubiquinone oxidoreductase. This complex is integral to maintaining cellular energy homeostasis as it catalyzes the initial step in oxidative phosphorylation. As part of the large multi-subunit Complex I MT-ND3 along with other subunits assists in proton translocation across the mitochondrial membrane which is indispensable for generating an electrochemical gradient used in ATP synthesis.
Pathways
The MT-ND3 subunit plays a role in the electron transport chain and oxidative phosphorylation pathways. It collaborates with other complex subunits like MT-ND1 and MT-ND2 to facilitate electron flow and proton pumping. These pathways are central to cellular respiration influencing how cells extract energy from nutrients. MT-ND3 is indirectly involved with other proteins like cytochrome c which further participates in the cascade of electron transfer leading to ATP production.
Mutations in the MT-ND3 gene have been linked to mitochondrial disorders such as Leber's hereditary optic neuropathy (LHON) and mitochondrial encephalomyopathy. These conditions often manifest due to disrupted oxidative phosphorylation leading to reduced ATP availability and increased reactive oxygen species production. MT-ND3 dysfunction might also impact other mitochondrial components like MT-CO1 as these proteins collectively impact energy production and cellular health.