Abcam, ab193353, Anti-DNA Ligase IV/LIG4 antibody [EPR16531]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal DNA Ligase IV/LIG4 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 20 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16531,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DNA Ligase IV also known as LIG4 or DNA Ligase 4 is an enzyme with a molecular mass of approximately 103 kDa. It is known to play a vital role in the DNA repair process by catalyzing the final step of DNA double-strand breaks where it joins two DNA strands in a phosphodiester bond. DNA Ligase IV is widely expressed in human tissues and its activity is key in the non-homologous end joining (NHEJ) pathway which repairs double-strand breaks in DNA. This protein forms a multi-protein complex with its cofactor XRCC4 and sometimes with other accessory proteins for efficient functioning.
Biological function summary
The significance of DNA Ligase IV in maintaining genomic stability makes it essential for proper cellular functions. DNA Ligase IV is a part of the NHEJ repair complex and works in close association with proteins such as XRCC4 and XLF-Cernunnos which stabilize the complex. It orchestrates the repair of DNA damage that could otherwise lead to mutations or chromosomal translocations. Furthermore LIG4 is involved in V(D)J recombination a process critical for immune system development by creating diversity in immunoglobulins and T cell receptors.
Pathways
DNA Ligase IV plays a significant role in the NHEJ pathway which is one of the two main pathways for repairing DNA double-strand breaks. This pathway provides a rapid and essential means of maintaining DNA integrity. DNA-PKcs a kinase activates the NHEJ pathway and works closely with LIG4. Additionally LIG4 takes part in V(D)J recombination orchestrating the proper joining of DNA segments important for developing a functional immune system. The protein Ku is also essential in these pathways acting as a scaffold for the entire repair machinery.
Defects or mutations in DNA Ligase IV can lead to severe health issues such as LIG4 syndrome a condition characterized by immunodeficiency and developmental delay and increased radiation sensitivity. Another disorder associated is leukemia as faulty NHEJ can result in chromosomal translocations leading to cancer. The interaction with XRCC4 is necessary for its DNA repair tasks and mutations affecting this interaction can exacerbate the disorder's severity. Understanding the role of DNA Ligase IV in these contexts provides insight into potential therapeutic targets for managing such conditions.