Abcam, ab193713, Human RANK ELISA Kit

Size: 1 x 96Tests
Human RANK ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human RANK in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.4 ng/mL,
Range:0.4 - 100 ng/mL,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Abcam's RANK Human ELISA Kit (ab193713) is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human RANK in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human RANK coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures RANK present in the samples. The wells are washed and biotinylated anti-Human RANK antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of RANK bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C