Abcam, ab193726, Mouse MDC ELISA Kit (Macrophage Derived Chemokine)

Size: 1 x 96Tests
Mouse MDC ELISA Kit (Macrophage Derived Chemokine) is a Sandwich (quantitative) ELISA for the measurement of Mouse MDC (Macrophage Derived Chemokine) in Mouse in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 2 pg/mL,
Range:2 - 600 pg/mL,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Abcam's MDC (Macrophage Derived Chemokine) Mouse ELISA Kit (ab193726) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MDC in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse MDC coated onto a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures MDC present in the sample. The wells are washed and a biotinylated anti-mouse MDC antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted into the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of MDC bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MDC also known as CCL22 or macrophage-derived chemokine is a small protein involved in immune system signaling. The molecular mass of MDC is approximately 8 kDa. It is expressed in various tissues including lymph nodes thymus and lungs where it mainly acts to regulate immune cell movement. Researchers commonly study this target using MDC assays and CCL22 ELISA to better understand its functions and expression patterns.
Biological function summary
MDC interacts with immune cells to mediate responses such as chemotaxis. It binds to the CCR4 receptor which is found on T cells and other immune cells. MDC does not require complex formation to function; it works independently to attract specific cell types. This chemokine provides a way for immune cells to navigate to sites of inflammation or infection.
Pathways
MDC plays a significant role in inflammatory and immune response pathways. It is integrally linked with the chemokine signaling pathway where it works along with other chemokines to guide immune responses. MDC is related to proteins like CCR4 and other chemokines in these pathways facilitating communication between cells during immune responses and maintaining homeostasis.
MDC is implicated in conditions such as allergic responses and autoimmune diseases. Its interaction with CCR4 connects it to disorders like asthma and atopic dermatitis where excessive immune cell recruitment leads to inflammation. Studying MDC levels through macrophage assays and chemokine ELISAs can provide insights into its role in these diseases and help develop potential therapeutic strategies.