Abcam, ab194585, Anti-CARD8 antibody - N-terminal

Size: 100µL
Rabbit Polyclonal CARD8 antibody. N-terminal. Suitable for WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CARD8 aa 1-200.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human CARD8 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.Q9Y2G2

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CARD8 also known as TUCAN or CARDINAL is a member of the CED-4 family of apoptosis proteins. This protein has a molecular mass of approximately 48 kDa. CARD8 expresses mostly in immune cells like monocytes and lymphocytes. Mechanically it possesses a caspase recruitment domain (CARD) which engages in protein-protein interactions. CARD8 interacts with components of the inflammasome playing a role in regulating apoptosis and inflammatory responses.
Biological function summary
CARD8 serves as a regulatory component in the innate immune system. It belongs to the NLR family and forms part of the inflammasome complex. Inflammasomes are essential for activating pro-inflammatory cytokines like IL-1β. CARD8 modulates the activity of caspase-1 controlling cytokine maturation and secretion. By inhibiting the activation of these pro-inflammatory cytokines CARD8 affects the immune response during infections and inflammatory conditions.
Pathways
CARD8 is involved in both the inflammasome and apoptosis pathways. In the inflammasome pathway CARD8 interacts with proteins like NLRP3 and ASC to regulate caspase-1 activation. In the apoptosis pathway it connects with other proteins like caspase-9 influencing cell death processes. The integration into these pathways highlights CARD8's role in balancing immune response and cell survival.
CARD8 has links to various inflammatory diseases and cancers. CARD8 impacts ailments such as rheumatoid arthritis and certain types of cancer by its ability to influence apoptosis and inflammation. In rheumatoid arthritis altered CARD8 function can lead to excessive inflammation partly through its connection with NLRP3 and caspase-1. In cancer its dysregulation impacts tumor survival pathways potentially linking it with other proteins like caspase-9.