Abcam, ab195215, Human IgG ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests
Human IgG ELISA kit is a single-wash, 90-minute SimpleStep ELISA® assay designed for the quantification of human Immunoglobulin G (IgG) with a sensitivity of 0.02 ng/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric sandwich ELISA - 450nm readout; works on any plate reader - High-throughput options – available in 96-well and 384-well formats, supporting large-scale sample processing. - Broad sample compatibility – works with serum, plasma, cell culture supernatants, and other biological fluids - Cited in over 20 publications
Key facts
Detection method:Colorimetric,
Sample types:Tissue Lysate, Cerebral Spinal Fluid, Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.02 ng/mL,
Range:0.23 - 15 ng/mL,
Assay time:1h 30m,
Assay Platform:Microplate

Product details:
Human IgG ELISA Kit ab195215 is a rapid single-wash 90-min ELISA kit to measure Human IgG in tissue lysate, cerebral spinal fluid, saliva, urine, cell culture supernatant, milk, serum and plasma. This SimpleStep ELISA® sensitivity is 0.02 ng/mL.
How the assay works
Human IgG SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Human IgM, human IgA and human IgE were prepared at 10 ng/mL and 250 ng/mL in Sample Diluent NS and assayed for cross reactivity. No cross-reactivity was observed for IgM or IgE at either concentration with a mean OD deviation from background of -0.01. No cross-reactivity was observed for IgA at 10 ng/mL and only 0.4% cross-reactivity at 250 ng/mL of IgA. Purified human IgG was assayed at 5 ng/mL in the presence and absence of 250 ng/mL of human IgM, human IgA and human IgE to determine interference. After background subtraction, recovery of human IgG was observed at a mean of 98% with a standard deviation of 0.06.
Human IgG ELISA Kit ab195215 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human IgG ELISA Kit ab195215
Human IgG ELISA Kit ab195215 has been used to study human immunoglobulin g in autoimmune diseases and infections in various physio-pathological contexts such as obesity
and SARS-CovV-2
References:
Leonid Kagan et al. 2023, PMID: 36949370,
Peter D Sun et al. 2023, PMID: 37821447
Save your precious samples
Our 384-well Human IgG ELISA Kit only requires a maxmum of 12.5µL
Related and recommended products
Human IgG ELISA Kit ab195215 is commonly used to measure immune system functioning.
Other ELISA kits to measure Immunoglobulins include:
- Human IgM ELISA kit
ab214568
- Human IgA ELISA kit
ab196263
- Human IgD ELISA kit
ab320794
- Human IgE ELISA kit
ab195216
- Human IgG ELISA Kit (10 x 96 well plate)
ab212169
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Immunoglobulin G (IgG) serves as an essential component of the immune system functioning mainly as an antibody. It is found in blood and extracellular fluid making it one of the most prevalent antibodies in the human body. IgG weighs approximately 150 kDa and it is expressed by B cells as part of the humoral response to pathogens. Alternate names include gamma globulin and Ig gamma. IgG antibodies form a critical aspect of adaptive immunity by targeting specific antigens for neutralization or destruction.
Biological function summary
IgG participates in several immune processes by binding to antigens and forming immune complexes. It facilitates pathogen opsonization initiating phagocytosis by immune cells like macrophages and neutrophils. IgG also activates the classical complement pathway which enhances pathogen clearance. IgG exists in a monomeric form and can cross the placenta providing passive immunity to the fetus. In laboratory settings its high specificity makes it valuable in techniques like IgG ELISA for detecting antigen presence.
Pathways
IgG plays an integral role in the complement system and the adaptive immune response. It partners with proteins such as C1q to initiate the classical complement pathway amplifying the body's ability to detect and remove pathogens. IgG also interacts with Fc receptors on immune cells modulating immune cell activity and facilitating antigen presentation to T cells. These interactions enhance the immune system's efficiency in recognizing and responding to diverse pathogens.
IgG has connections to autoimmune diseases and chronic infections. Elevated levels of IgG can indicate autoimmune conditions like lupus or rheumatoid arthritis where the immune system mistakenly attacks healthy tissue. During chronic infections such as HIV variations in IgG responses can affect disease progression and treatment efficacy. In these contexts IgG interacts with proteins involved in the immune dysregulation characteristic of such diseases highlighting its significant role in both protection and pathology.