Abcam, ab196263, Human IgA ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests / 10 x 96Tests
Human IgA ELISA kit is a single-wash 90-min SimpleStep ELISA used to quantification of human Immunoglobulin A (IgA) with a sensitivity of 0.25 ng/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric sandwich ELISA - 450nm readout; works on any plate reader - Different formats for different needs: 384-well for higher throughput - Broad sample compatibility – works with saliva, urine, plasma, cell culture supernatants, milk, and serum - Cited in over 10 publications
Key facts
Detection method:Colorimetric,
Sample types:Saliva, Urine, Plasma, Cell culture supernatant, Milk, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.25 ng/mL,
Range:0.78 - 50 ng/mL,
Assay time:1h 30m,
Assay Platform:Microplate

Product details:
Human IgA ELISA Kit ab196263 is a rapid single-wash 90-min ELISA kit to measure Human IgA in saliva, urine, plasma, cell culture supernatant, milk, serum. This SimpleStep ELISA® sensitivity is 0.25 ng/mL.
How the assay works
Human IgA SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and purified Human IgA protein in serum, plasma, milk, urine, saliva, and cell culture media samples only. Cell and tissue extracts have not been tested with this kit.
Human IgA ELISA Kit ab196263 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human IgA ELISA Kit ab196263
Human IgA ELISA Kit ab196263 has been used to study inflammatory responses to acute pancreatitis
and to gastric bypass surgery in obese patients
References:
Yuhan Wang et al. 2022, PMID: 35273695,
Xiaojiang Dai et al. 2017, PMID: 28222218.
Save your precious samples
Our 384-well Human IgA ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human IgA Antibody Pair - BSA and Azide free
ab242035
Related and recommended products
Human IgA ELISA Kit ab196263 is commonly used to measure immune system functioning.
Other ELISA kits to measure Immunoglobulins include:
- Human IgG ELISA kit
ab195215
- Human IgM ELISA kit
ab214568
- Human IgD ELISA kit
ab320794
- Human IgE ELISA kit
ab195216
Human Immunoglobulin A (IgA) belongs to the immunoglobulin family of proteins which include Human IgG, IgE, and IgM, and are known as antibodies. IgA has an essential role in mucosal immunity. IgA is the second most common serum Ig and is about one-fifth of the concentration of IgG, however it is the most abundant immunoglobulin in secretions, such as saliva, mucus, sweat, and tears. In normal human serum, IgA is found mainly as a monomeric, alternatively, in secretions, IgA is found as a dimer containing two additional proteins, secretory piece, and a J chain. The secretory piece is synthesized in the epithelial cells and is added to IgA as it passes into the secretions, protecting it from degradation. IgA works is with lysozymes to hydrolyze carbohydrates in bacterial cells walls to clear the infection. The most common disease associated with high levels of IgA in the kidney is IgA nephropathy, aka Berger's disease. There is no clear clarification as to why IgA deposits occur in the kidney, however abnormality in the immune system is a possibility. Other diseases that IgA is associated with are Celiac disease and Henoch-Schonlein purpura (HSP).

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C