Abcam, ab196413, Recombinant human EZH1 + EED + SUZ12 + RBBP4 + AEBP2 protein (Active)

Size: 50µg
Recombinant human EZH1 + EED + SUZ12 + RBBP4 + AEBP2 protein (Active) is a Human Full Length protein, in the 2 to 425 aa range, expressed in Baculovirus infected Sf9 cells, with >79%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>79% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:Tag free,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:50 μl reaction mix (20 mM phosphate pH 7.4, 0.05% Tween-20, 40 μM S-adenosylmethionine, and ab196413 were added to the wells coated with the substrate on a Neutravidin white plate. Incubated for 1 hr. Added antibody against methylated K27 residue of histone H3, incubated 1 hr. Then, added secondary HRP-labeled antibody and incubated 30 min. Finally, added HRP chemiluminescent substrates and read luminescence.,
Accession:Q09028,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Preservative: 1.36% ImidazoleConstituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.02% Potassium chloride

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The EZH1 EED SUZ12 RBBP4 and AEBP2 proteins form the core of Polycomb Repressive Complex 2 (PRC2) which is an essential multi-subunit protein assembly. The main function of PRC2 lies in its ability to methylate histone H3 on lysine 27 (H3K27me3) an important histone modification promoting chromatin compaction and gene silencing. The molecular weight of each subunit varies with EZH1 around 85 kDa. The PRC2 components such as EZH1 mostly express in tissues where epigenetic regulation is required including embryonic stem cells and various somatic tissues.
Biological function summary
This complex plays a significant role in maintaining transcriptional repression across the genome contributing to cellular identity and stem cell pluripotency. PRC2 including the catalytic subunit EZH1 and its accessory proteins EED SUZ12 RBBP4 and AEBP2 facilitates chromatin remodeling and maintenance of gene silencing by regulating the histone methylation status. This biological mechanism influences differentiation pathways and is important in preserving a balance between cell proliferation and specialization.
Pathways
EZH1 and its associated complex modulate important developmental and signaling pathways such as the Wnt and Notch pathways. PRC2 works closely with other chromatin modifiers and regulators like HDAC1/2 contributing to the fine-tuning of gene expression during development and cellular differentiation. These pathways are integral to the regulation of cell fate decisions and PRC2's activity ensures proper pathway signaling through chromatin-mediated control.
Abnormalities in EZH1 and associated PRC2 components link to certain cancers especially leukemia and breast cancer. Mutations or altered expression levels of PRC2 subunits influence chromatin structure and gene regulation potentially leading to oncogenesis. These components interact with other proteins such as DNMT3A and TET2 which also play roles in epigenetic modifications and cancer development. Understanding the relationship between PRC2 and disease offers potential therapeutic avenues for targeting these pathways in cancer treatment.