Abcam, ab197001, Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric)

Size: 100Test
Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (ab197001) provides a quick and easy way for monitoring the activity of triose phosphate isomerase (TPI) in a variety of samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Tissue, Suspension cells, Serum, Adherent cells,
Assay type:Enzyme activity,
Sensitivity:> 40 mU/mL,
Assay time:30m,
Assay Platform:Microplate reader

Product details:
Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (ab197001) provides a quick and easy way for monitoring the activity of triose phosphate isomerase (TPI) in a variety of samples. In this kit, triose phosphate isomerase (TPI) converts dihydroxyacetone phosphate into glyceradehyde-3-phosphate, which reacts with the enzyme mix and developer to form a colored product with strong absorbance at OD=450 nm.
The assay is simple, sensitive and high-throughput and can detect TPI activity as low as 40 mU/mL.
TPI assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader every 2-3 min for 20-40 min
This product is manufactured by BioVision, an Abcam company and was previously called K670 Triose Phosphate Isomerase (TPI) Activity Colorimetric Assay Kit. K670-100 is the same size as the 100 test size of ab197001.
Triose Phosphate Isomerase (TPI, TIM, EC 5.3.1.1) is an important enzyme for glycolysis. It catalyzes the reversible conversion of dihydroxyacetone phosphate to glyceraldehyde-3-phosphate, thus maintaining the equilibrium of these two triose phosphates. TPI connects glycolysis to pentose phosphate pathway and lipid metabolism. It is a stable homodimer found in almost all organisms.
In humans, TPI deficiency is a rare multisystem disorder and leads to progressive neurological dysfunction, characterized by hemolytic anemia, cardiomyopathy and progressive neuromuscular impairment.
REACH authorisation
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Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Triosephosphate isomerase also known as TPI is an enzyme that plays an important role in glycolysis and gluconeogenesis by catalyzing the reversible isomerization of dihydroxyacetone phosphate (DHAP) to glyceraldehyde 3-phosphate (G3P). It has a molecular mass of approximately 27 kDa. This enzyme is widely expressed in many tissues with high presence in red blood cells muscle and brain tissues due to their high energy demands. As a dimer enzyme it exhibits significant catalytic efficiency that supports cellular energy management.
Biological function summary
The enzyme facilitates the efficient production of ATP by maintaining balance between DHAP and G3P vital for energy-metabolizing cells. Triosephosphate isomerase is not part of a larger complex itself but it significantly interacts with other enzymes in the glycolytic pathway. This interaction supports cellular respiration by ensuring a steady supply of intermediates needed for downstream processes.
Pathways
Triosephosphate isomerase is central to glycolysis and gluconeogenesis pathways. In glycolysis it works in tandem with other enzymes such as hexokinase and phosphofructokinase to breakdown glucose into pyruvate yielding ATP and NADH. It provides a critical link between glycolysis and energy production processes impacting the cellular metabolism and biochemical energy cycles. The enzyme also contributes to gluconeogenesis where it helps synthesize glucose from non-carbohydrate sources ensuring energy supply during fasting.
Mutations or deficiencies in triosephosphate isomerase can lead to conditions such as triosephosphate isomerase deficiency a rare autosomal recessive disorder characterized by progressive neurological dysfunction hemolytic anemia and muscle weakness. This deficiency arises from dysfunctional or unstable isomerase activity leading to an accumulation of DHAP. Additionally research has shown links between this enzyme and Alzheimer's disease where altered glucose metabolism is a common feature. Abnormal triosephosphate isomerase activity may interact with amyloid precursor protein processing in the disease pathology.