Abcam, ab197011, Glutamine Assay Kit (Colorimetric)

Size: 100Test
Glutamine Assay kit (Colorimetric) ab197011 is a simple and sensitive assay to quantify the concentration of glutamine in biological fluids and tissues. - Cited in >35 Publications - Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
Detection method:Colorimetric,
Sample types:Urine, Plasma, Tissue, Serum, Other biological fluids,
Assay type:Quantitative,
Sensitivity:= 25 µM,
Assay time:90m,
Assay Platform:Microplate reader

Product details:
How the assay works
The glutamine assay protocol is based on the hydrolysis of Glutamine to Glutamate producing a stable colored signal, which is directly proportional to the amount of glutamine in the sample.
Glutamine assay protocol summary:
- Add samples and standards to wells
- Add hydrolysis mix and incubate for 30 min
- Add reaction mix and incubate for 60 min
- Analyze with a microplate reader.
How other researchers are using
Glutamine Assay Kit (Colorimetric) has been used in a variety of sample type including:
Human eutopic endometrial and ectopic endometrial tissues
Humanpancreatic cancer cell lines (PANC-1 and Capan-1)
Human Esophageal squamous cell carcinoma (ESCC) cell lines
References:
1-Wu K et al. 2024
2-Kim M et al. 2023
3-Chen L et al. 2023
This product is manufactured by BioVision, an Abcam company and was previously called K556 Glutamine Colorimetric Assay Kit. K556-100 is the same size as the 100 test size of ab197011.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glutamine often abbreviated as Gln is an amino acid with a molecular weight of approximately 146.15 g/mol. It serves as a significant nitrogen donor in cellular processes and contributes to synthesizing nucleotides and amino acids. The amino acid is non-essential meaning the body can synthesize it as needed mainly produced in muscles and distributed via the bloodstream to other tissues including the liver brain and immune cells. Glutamine's role in ammonia transportation is notable as it helps in detoxifying ammonium ions. It influences cellular proliferation through its involvement in various cellular functions.
Biological function summary
Glutamine plays an important role in supporting rapid cell division and growth such as in the immune system and intestinal tract. It forms part of the larger metabolic function known as glutaminolysis which provides energy and precursors for biosynthesis. Within cells glutamine works in tandem with several enzymes and transport proteins such as glutamine synthetase which regulates its production and use. The connection with the liver is significant as the organ converts glutamine into glucose and ammonium with glutamate acting as an intermediary.
Pathways
The role of glutamine extends to important areas like the tricarboxylic acid (TCA) cycle and the glutamate-glutamine cycle. In the TCA cycle it serves as a carbon source feeding into gluconeogenesis and other biosynthetic pathways. The conversion to glutamate followed by other downstream metabolites links glutamine closely to amino acid metabolism. Important proteins like glutamate dehydrogenase facilitate these conversions and highlight the interconnected nature of these metabolic pathways.
Abnormalities in glutamine metabolism have associations with conditions such as cancer and neurodegenerative diseases. Cancer cells often exhibit increased glutamine uptake supporting their rapid growth through metabolic pathways like glutaminolysis. On the other hand neurodegenerative diseases involve disruptions in the glutamate-glutamine cycle implicating excitotoxicity-related proteins like NMDA receptors. Altered levels of glutamine and associated proteins in these diseases suggest potential therapeutic targets and diagnostic markers emphasizing its role in cellular health and disease progression.