Abcam, ab197025, Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal 2 Hydroxy phytanoyl CoA lyase/HACL1 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16812,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
2-Hydroxyphytanoyl-CoA lyase (HACL1) also known as phytanoyl-CoA hydroxylase is an enzyme that plays a mechanical role in breaking down branched-chain fatty acids specifically phytanic acid. HACL1 catalyzes the cleavage of 2-hydroxyphytanoyl-CoA to pristanal producing formyl-CoA in the process. This enzyme has a molecular mass of approximately 50 kDa. HACL1 is mainly expressed in peroxisomes which are subcellular compartments involved in lipid metabolism.
Biological function summary
2-Hydroxyphytanoyl-CoA lyase serves a critical function in the metabolism of fatty acids derived from dietary sources especially chlorophyll-rich plant materials. It operates as part of the α-oxidation pathway a unique metabolic route for the degradation of phytanic acid which is a type of branched-chain fatty acid that cannot be processed by β-oxidation. HACL1 is not known to be part of a larger enzyme complex unlike some other enzymes involved in fatty acid metabolism.
Pathways
The α-oxidation pathway is where HACL1 plays its significant role. This pathway starts with the conversion of phytanic acid into 2-hydroxyphytanoyl-CoA which HACL1 then cleaves. The α-oxidation pathway connects to the broader lipid metabolism processes in the body. Related proteins involved in these pathways include fatty aldehyde dehydrogenase (FALDH) which further processes the metabolic products of HACL1 activity.
Disruptions in the function of HACL1 can lead to Refsum disease which is characterized by the accumulation of phytanic acid in the body resulting in neurological symptoms. HACL1's role can be connected to proteins involved in peroxisomal functions like peroxisome biogenesis factor proteins whose dysfunction might also contribute to metabolic disorders similar to Refsum disease such as Zellweger spectrum disorders.