Abcam, ab197031, Anti-U2AF65 antibody [EPR17046] - C-terminal

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal U2AF65 antibody. C-terminal. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 5 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR17046,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
U2AF65 also known as U2 small nuclear RNA auxiliary factor 2 (U2AF2) is a splicing factor structured around 65 kilodaltons in mass. It interacts with RNA as part of the spliceosome to facilitate proper pre-mRNA splicing. The protein ensures the precise recognition and binding of the polypyrimidine tract at the 3' splice site. It is expressed in various human tissues playing an important role in RNA processing across different cell types.
Biological function summary
The U2AF65 protein contributes to the assembly of the spliceosome a major RNA-protein complex involved in splicing pre-messenger RNA. It works as part of the heterodimeric U2AF complex along with U2AF35 enhancing the precise selection of splice sites. This activity is essential to ensure accurate and efficient splicing which is necessary for proper gene expression and regulation.
Pathways
The activity of U2AF65 is an integral part of the RNA splicing pathway which plays a critical role in modulating gene expression. U2AF65 acts in coordination with other splicing factors such as SF1 and SF3B1 ensuring the correct assembly of the functional spliceosome. The accurate regulation of this pathway is essential for generating diversity in protein expression and for maintaining cell homeostasis.
Misregulation of U2AF65 activity has implications in diseases like cancer and spinal muscular atrophy. Alterations in the expression or function of U2AF65 can lead to aberrant splicing events which affect gene expression profiles. In cancer U2AF65 interacts with various oncogenes and tumor suppressor proteins potentially contributing to tumour progression. In spinal muscular atrophy alterations in splicing factors including U2AF65 influence the expression of survival motor neuron proteins intricately linking U2AF65 to the pathology of these conditions.