Abcam, ab197742, Mouse IL-1 beta ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests
Mouse IL-1 beta ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify mouse interleukin-1 beta (IL-1β) with a sensitivity of 1 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric sanwich ELISA – 450 nm readout, compatible with any standard plate reader - Available in different formats – 384-well format available for high-throughput applications - Broad sample compatibility – works with serum, citrate plasma, urine, cell culture extracts, tissue extracts, and cell culture supernatant - Cited in over 200 publications
Key facts
Detection method:Colorimetric,
Sample types:Cell culture extracts, Cell culture supernatant, Citrate plasma, Serum, Tissue Extracts, Urine,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 1 pg/mL,
Range:1.56 - 100 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Mouse IL-1 beta ELISA Kit ab197742 is a rapid single-wash 90-min ELISA kit to measure cell culture extracts, cell culture supernatant, tissue extracts, urine, serum and plasma. This SimpleStep ELISA® sensitivity is 1 pg/mL.
How the assay works
SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant mouse IL-1 beta protein in serum, plasma, and cell culture supernatant, cell and tissue extract samples only. Mouse brain, kidney, liver and lung homogenates were tested but no signal was detected. Plasma (EDTA) and plasma (heparin) samples have not been tested with this kit.
Mouse IL-1 beta ELISA Kit ab197742 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Mouse IL-1 beta ELISA Kit ab197742
Mouse IL-1 beta ELISA Kit ab197742 has been used to study inflammation in various physio-pathological contexts such as liver disease
, diabetes
, and Alzheimer's Disease
References:
Ai-Lei Xu et al. 2024, PMID: 37902450,
Lili Shi et al. 2023, PMID: 38169591,
Guodong Huang, et al. 2023, PMID: 36325883
Save your precious samples
Our 384-well Mouse IL-1 beta ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Mouse IL-1beta Antibody Pair - BSA and Azide free
ab241673
- Anti-IL-1 beta antibody [EPR16805-1] - BSA and Azide free (Capture)
ab242452
- Anti-IL-1 beta antibody [EPR16805-57] - BSA and Azide free (Detector)
ab242703
Related and recommended products
Mouse IL-1 β ELISA kit ab197742 is commonly used to measure IL-1 beta as a marker of inflammation.
Other ELISA kits to measure inflammatory markers include:
- Mouse IL-6 ELISA kit
ab222503
- Mouse TNF alpha ELISA kit
ab208348
- Mouse IL-18 ELISA kit
ab216165
IL-1 beta (IL-1β) is a 17.5 kDa cytokine protein of the Interleukin 1 family. IL-1β is secreted by macrophages and other cell types in response to inflammatory agents or infection. IL-1β plays a role in a number of cellular processes, including immune responses, bone remodeling, apoptosis and inflammatory pain hypersensitivity.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C