Abcam, ab197750, Mouse SCF ELISA Kit

Size: 1 x 96Tests
Mouse SCF ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse SCF with a sensitivity of 1.11 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Cell culture supernatant, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.11 pg/mL,
Range:15.63 - 1000 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Mouse SCF ELISA Kit (ab197750) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SCF protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse SCF with 1.11 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Stem Cell Factor (SCF), also known as c-kit ligand, mast cell growth factor (MGF), and Steel Factor, is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. Mutations in either SCF or its receptor, c-kit tyrosine kinase receptor, have the same complex phenotype that affects hematopoiesis, gametogenesis, and melanogenesis. SCF stimulates the proliferation of mast cells and is able to augment the proliferation of both Myeloid and lymphoid hematopoietic progenitors in bone marrow culture. Additionally, fibroblasts, bone marrow stromal cells, macrophages, astroglia, and neurons widely express mouse SCF.Mouse SCF cDNA encodes a 248 amino acid (aa) residue. This residue is a type I transmembrane protein containing a 25 aa signal peptide, a 189 aa extracellular region, a 23 aa TM domain, and a 36 aa cytoplasmic tail. Through alternative splicing, two isoforms of cell-associated SCF exist: KL-1 and KL-2. These isoforms differ by the presence or absence of exon 6, which encodes a proteolytic cleavage site. The 164-165 aa secreted soluble form of SCF is generated by proteolytic cleavage from KL-1. Human SCF and rat SCF are 80% and 95% identical to mouse SCF, respectively.The standard is affinity purified.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Stem Cell Factor (SCF) also known as kit ligand c-kit ligand or KITLG is a critical protein involved in cell signaling. It usually appears as a 30 kDa glycoprotein. SCF is widely expressed in various tissues including the bone marrow brain and fetal liver. Its major mechanical function is to bind to the c-KIT receptor triggering various intracellular pathways that influence cell proliferation survival and differentiation. Aside from its other names SCF in mouse plasma is often monitored including in assays like c-kit ligand ELISA.
Biological function summary
SCF acts as an indispensable growth factor promoting hematopoiesis and melanogenesis. It plays a significant role in the regulation of hematopoietic stem cells and mast cells. SCF and its receptor c-KIT form a complex that activates signaling pathways essential for these processes. This protein is also involved in gametogenesis by aiding the proliferation and survival of primordial germ cells. SCF's action is not limited to mammals as studies demonstrate its activity in other species like mouse SCF.
Pathways
SCF impacts multiple important biological systems by engaging with c-KIT. The SCF/c-KIT interaction contributes to the MAPK/ERK pathway influencing cell division and growth. It also plays a role in the PI3K/AKT pathway that governs cell survival. Beyond its direct association with c-KIT SCF's involvement in these pathways connects it to several other proteins like RAS and PI3K which are important for signal transduction.
SCF has associations with several pathological conditions. Dysregulation of the SCF/c-KIT pathway can lead to disorders like mastocytosis and certain leukemias. The overactivation or mutations in c-KIT are often implicated in these diseases due to its cooperative action with SCF. Additionally aberrant SCF signaling can contribute to the progression of gastrointestinal stromal tumors (GIST) where c-KIT has known relevance. Understanding SCF's role can therefore provide insights into diagnostic and therapeutic strategies for these conditions.