Abcam, ab19862, Anti-SCD1 antibody [CD.E10]

Size: 100µg
Anti-SCD1 antibody [CD.E10] (ab19862) is a mouse monoclonal antibody detecting SCD1 in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Human . - KO validated for confirmed specificity - Over 80 publications - Trusted since 2005
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:CD.E10,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt, WB, IP, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human SCD.O00767,
Specificity:This SCD1 antibody can bind to SCD5 very weakly in WB when SCD5 is overexpressed (<10% cross-reactivity).

Product details:
What is this antibody validated in?
Anti-SCD1 antibody [CD.E10] (ab19862) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of SCD1?
Anti-SCD1 [CD.E10] (ab19862) specifically detects a band for SCD1 (UniProt: O00767) at a molecular weight of 42kDa.
Trusted by the scientific community
Anti-SCD1 [CD.E10] (ab19862) was first used in a scientific publication in 2005 and has been cited over 80 times in peer-reviewed journals.
Reviewed by scientists
Anti-SCD1 [CD.E10] (ab19862) has over 5 independent reviews from customers.
Specificity confirmed
The specificity of Anti-SCD1 antibody [CD.E10] (ab19862) has been confirmed by Western blot testing in SCD Knockout HeLa cell line,
ab265220

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Protein A affinity chromatography, Storage buffer-pH: 7.2 Preservative: 0.05% Sodium azide Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Stearoyl-CoA desaturase-1 (SCD1) also known as delta-9-desaturase is an enzyme integral to the synthesis of monounsaturated fats. This enzyme converts saturated fatty acyl-CoAs to monounsaturated fatty acyl-CoAs by introducing a double bond between the ninth and tenth carbon atoms a process called desaturation. SCD1 has a molecular weight of approximately 45 kDa and expresses in a variety of tissues including the liver adipose tissue and heart.
Biological function summary
SCD1 plays a significant role in fatty acid metabolism by regulating the balance of saturated and unsaturated fatty acids which impacts membrane fluidity and cellular signaling. SCD1 does not function as part of a multi-protein complex acting independently in the endoplasmic reticulum. Its expression and activity influence lipid biosynthesis and storage affecting energy homeostasis and lipid composition in cells.
Pathways
Fatty acid synthesis and oxidation pathways extensively involve SCD1. It participates mainly in the lipogenesis pathway interacting with proteins like acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS). SCD1 contributes to maintaining the ratio of oleate and palmitoleate key monounsaturated fatty acids important for cellular functions by integrating into the lipid metabolism network.
SCD1 is implicated in conditions like obesity and metabolic syndrome. Altered SCD1 activity links to insulin resistance as seen in obesity by modulating lipid profiles that influence insulin sensitivity. SCD1 associates with proteins like peroxisome proliferator-activated receptor gamma (PPARγ) which regulates adipogenesis emphasizing its role in metabolic disorders.