Abcam, ab198912, Anti-n-Myc/MYCN antibody

Size: 100µL
Rabbit Polyclonal N-Myc/MYCN antibody. Suitable for IHC-P and reacts with Human, Mouse, Rat samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human MYCN.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MYCN. The exact immunogen used to generate this antibody is proprietary information.P04198

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.05% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
N-Myc also known as MYCN is a transcription factor encoded by the MYCN gene. It plays an important role in cell proliferation and differentiation. The n-Myc protein is part of the Myc family of transcription factors including c-Myc and c-Myb. n-Myc has a molecular weight of approximately 64 kDa. This protein expresses predominantly in developing tissues particularly in the brain but its expression is seen in other tissues during embryogenesis as well. This characteristic makes it important in early development and organ formation.
Biological function summary
N-Myc/MYCN functions in gene regulation by binding to E-box sequences in DNA facilitating transcription of genes essential for cellular growth and metabolism. n-Myc often forms a heterodimer with the MAX protein which enhances its DNA-binding capacity and transcriptional activity. This activity influences processes like apoptosis cell differentiation and cell cycle progression. The ncm mouse model a genetically engineered mouse with MYCN overexpression demonstrates the effects of deregulated n-Myc expression.
Pathways
MYCN significantly impacts the p53 signaling pathway and the Wnt signaling pathway. In the p53 signaling pathway n-Myc interacts with p53 and MDM2 regulating cell cycle checkpoints and apoptosis. In the Wnt pathway MYCN interplays with molecules like β-catenin to influence cell division and differentiation. These interactions highlight the role of n-Myc in maintaining cellular homeostasis and response to external signals that affect growth and division.
N-Myc is closely linked to neuroblastoma and medulloblastoma aggressive forms of pediatric cancers. MYCN amplification often indicates a poor prognosis in neuroblastoma playing a role in tumor progression and resistance to therapy. In medulloblastoma overexpression of n-Myc partners with proteins like Aurora A kinase to promote oncogenesis. Targeting these protein interactions offers potential therapeutic strategies for these MYCN-related cancers.