Abcam, ab199079, Mouse Clusterin ELISA Kit (Apolipoprotein J)

Size: 1 x 96Tests
Mouse Clusterin ELISA Kit (Apolipoprotein J) is a single-wash 90-min Simplestep used to quantify Mouse Clusterin (Apolipoprotein J) with a sensitivity of 280 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Urine, Citrate plasma, Cell culture supernatant, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 280 pg/mL,
Range:0.78 - 50 ng/mL,
Assay time:1h 30m,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Mouse Clusterin ELISA Kit (Apolipoprotein J) (ab199079) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Clusterin (Apolipoprotein J) protein in cell culture supernatant, cit plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Clusterin (Apolipoprotein J) with 280 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Clusterin (also known as Apoliprotein J) is a multifunctional protein, named for its ability to induce cellular clustering. The secreted form is processed from a single chain into alpha and beta strands, that form a heterodimeric, 80 kDa protein. Clusterin is also present as an alternatively spliced, 50 kDa intracellular form isoform. As an extracellular chaperone, Clusterin prevents the aggregation of blood plasma proteins and inhibits amyloid fibrils. Clusterin also plays a role in both apoptosis and proliferation. Secreted Clusterin protects against apoptosis by the complement system, but can promote apoptosis when present in the nucleus. Intracellular Clusterin interacts with ubiquitin and the proteasomal machinery for target degradation.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The biological target Apolipoprotein J also known as Clusterin or ApoJ is a glycoprotein with a mass of approximately 80 kDa. Clusterin is expressed in a wide range of tissues and bodily fluids including the brain liver and blood plasma. It acts as a chaperone helping to stabilize and transport proteins and lipids under physiological stress conditions. This protein also assists in inhibiting complement-mediated cell lysis thereby contributing to cell survival.
Biological function summary
The multifunctional role of Clusterin involves cell aggregation apoptosis and tissue remodeling. It does not act alone; Clusterin can integrate into complex formation with other proteins influencing various cellular processes. It partners with lipoproteins in blood influencing lipid transport and metabolism. Its prominent presence in extracellular fluids suggests a significant role in modulating immune responses and reducing oxidative stress.
Pathways
The involvement of Clusterin spans cholesterol metabolism and apoptotic pathways. It interacts importantly with key players like ApoE in cholesterol homeostasis impacting lipoprotein particle formation and hepatic lipid clearance. Additionally within apoptotic pathways Clusterin associates with Bcl-2 family proteins aiding in the regulation of cell death and survival signaling.
Apolipoprotein J correlates with Alzheimer's disease and certain cancers. In Alzheimer's Clusterin is upregulated associating with amyloid-beta plaques which implicates it in neurodegeneration processes. Additionally in cancer Clusterin connects with proteins like Bcl-xL influencing tumorigenesis and resistance to therapy. Its varied roles and interactions highlight its importance as a biomarker and potential therapeutic target.