Abcam, ab199081, Mouse IL-17A ELISA Kit

Size: 1 x 96Tests
Mouse IL-17A ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-17A with a sensitivity of 0.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair - Cited in over 10 citations
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Cell culture supernatant, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.5 pg/mL,
Range:6.25 - 400 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Mouse IL-17A ELISA Kit ab199081 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse IL-17A in cell culture supernatant, citrate plasma, serum. This SimpleStep sensitivity is 0.5 pg/mL.
How the assay works
Mouse IL-17A SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse IL-17A ELISA Kit ab199081 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-IL-17A antibody [EPR16663-61] - BSA and Azide free (Capture)
ab243016
- Anti-IL-17A antibody [EPR16663-89] - BSA and Azide free (Detector)
ab243016
IL-17A is a pro-inflammatory cytokine that is secreted by a subset of activated T cells. It is a disulfide-linked homodimer with both glycosylated and non-glycosylated forms. IL-17A induces stromal cells to produce pro-inflammatory and hematopoietic cytokines, and also enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C