Abcam, ab199084, Mouse M-CSF ELISA Kit

Size: 1 x 96Tests
Mouse M-CSF ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse M-CSF with a sensitivity of 3.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture extracts, Citrate plasma, Cell culture supernatant, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 3.7 pg/mL,
Range:46.9 - 3000 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Mouse M-CSF ELISA Kit (ab199084) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of M-CSF protein in cell culture supernatant, serum, cit plasma, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse M-CSF with 3.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
M-CSF is a secreted cytokine that plays an essential role in the regulation of differentiation and maintenance of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. It plays an important role in innate immunity and in inflammatory processes where it acts to promote the release of proinflammatory chemokines. M-CSF also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Reports show that it is required for normal male and female fertility. It also acts in reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
M-CSF also called CSF1 or macrophage colony-stimulating factor is a cytokine with a molecular mass of approximately 40 kDa. It plays a significant role in the regulation of macrophage production differentiation and function. M-CSF is expressed in various tissues including the bone marrow spleen and liver. The expression of this cytokine is pivotal for the development and survival of mononuclear phagocytes and is critical in myeloid lineage regulation.
Biological function summary
This cytokine functions as a growth factor that influences the survival proliferation and differentiation of hematopoietic progenitor cells into mature macrophages. M-CSF forms a homodimeric complex that interacts with its receptor c-Fms which is a cell surface glycoprotein expressed on monocytes and macrophages. This interaction is essential for signal transduction that leads to the activation of pathways involved in cell growth and proliferation. Unlike some other growth factors M-CSF does not require the presence of a co-factor to exert its effects on target cells.
Pathways
M-CSF is involved in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are critical for the mediation of cell proliferation and survival. Within these pathways M-CSF connects with other proteins such as AKT1 and MAPK1 which further transduce signals that aid in cellular growth and immune response regulation. M-CSF also interacts through its receptor c-Fms which propagates downstream signaling ensuring the appropriate activation of macrophage-related pathways.
An imbalance or dysregulation of M-CSF activity has been linked to disorders such as osteoporosis and certain cancers. Osteoporosis involves excessive bone resorption due to increased osteoclast activity where M-CSF participates in osteoclast differentiation. In cancer particularly breast and prostate cancer M-CSF expression supports tumor-associated macrophages promoting tumor progression. Additionally c-Fms is involved in these disease processes creating a significant therapeutic target for modulating M-CSF activity to potentially alleviate symptoms or halt disease progression.