Abcam, ab200486, Anti-TMEM16A antibody [DG1/447 + DOG-1.1]

Size: 500µL
Mouse Multiclonal TMEM16A antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ANO1 aa 900 to C-terminus.
Key facts
Host species:Mouse,
Clonality:Multiclonal,
Clone number:DG1/447 + DOG-1.1,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human ANO1. Database linkQ5XXA6

Product details:
What is an oligoclonal antibody?
This oligoclonal antibody is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: PBS, 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TMEM16A also known as ANO1 is a transmembrane protein functioning as a calcium-activated chloride channel. It plays a significant role in the regulation of membrane potential and fluid secretion. TMEM16A has a molecular mass of approximately 114 kDa. This protein is expressed in various tissues including airway epithelial cells intestinal epithelium and smooth muscle cells. Its expression is also noticeable in parts of the nervous system indicating its widespread physiological importance.
Biological function summary
TMEM16A contributes to processes such as epithelial fluid transport and smooth muscle contraction. It is an integral component of a calcium-sensitive chloride conductance. This channel regulates ion transport across epithelial cell membranes influencing fluid secretion in airways and gastrointestinal tracts. In blood vessels TMEM16A helps control vascular tone by mediating smooth muscle cell contraction which is dependent on intracellular calcium levels.
Pathways
TMEM16A interacts with key cellular signaling cascades especially the calcium signaling pathway. This interaction has critical implications for chloride conductance in epithelial cells and smooth muscle function. TMEM16A is linked with proteins like calcium/calmodulin-dependent protein kinase II (CaMKII) which further modulates its activity. Additionally TMEM16A is implicated in pathways regulating mucin secretion which involves other proteins like cystic fibrosis transmembrane conductance regulator (CFTR).
TMEM16A plays a role in respiratory diseases like asthma and cystic fibrosis where dysregulation in chloride channels leads to abnormal mucus secretion and airway obstruction. It also relates to hypertension due to its role in vascular tone modulation. Research shows TMEM16A interacts with proteins like CFTR in cystic fibrosis affecting chloride transport and fluid balance. In hypertension its interaction with calcium-sensing proteins further complicates vascular responsiveness and pressure regulation.