Abcam, ab200645, Anti-EDEM1 antibody

Size: 100µL
Rabbit Polyclonal EDEM1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human EDEM1 aa 150-200.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human EDEM1 aa 150-200. The exact immunogen used to generate this antibody is proprietary information.Q92611

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab200645 was purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)., Storage buffer-pH: 7.2Preservative: 0.097% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EDEM1 also known as ER degradation-enhancing alpha-mannosidase-like protein 1 is a protein that plays a role in the endoplasmic reticulum-associated degradation (ERAD) pathway. Its molecular mass is approximately 98 kDa. EDEM1 is expressed in various tissues but is highly present in the liver and pancreas. This protein helps to identify and target misfolded glycoproteins for degradation ensuring quality control within the endoplasmic reticulum.
Biological function summary
EDEM1 accelerates the removal of incorrectly folded proteins by promoting their retrotranslocation and subsequent degradation by the proteasome. It is not part of a stable complex but coordinates with ER chaperones to enhance the dislocation of substrates from the ER to the cytosol. EDEM1 regulates glycoprotein quality control helping to maintain proper protein homeostasis.
Pathways
EDEM1 contributes to the unfolded protein response (UPR) and the ERAD pathway. Both pathways are essential for managing protein folding and degradation within the cell. EDEM1 interacts with proteins like HERP and OS-9 during these processes helping with the recognition and processing of misfolded proteins. The correct functioning of these pathways is important for cellular adaptation to stress.
EDEM1's malfunction can link to conditions such as cystic fibrosis and neurodegenerative diseases. These diseases can arise due to defects in the clearance of misfolded proteins leading to their accumulation. EDEM1 interacts with proteins such as CFTR where improper folding is a known issue in cystic fibrosis. Its role in preventing the buildup of misfolded proteins makes it relevant to the pathology of these disorders.