Abcam, ab200993, Alexa Fluor® 647 Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377]

Size: 100µL
Rabbit Recombinant Monoclonal Topoisomerase II alpha antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5377,
Isotype:IgG,
Conjugation:Alexa Fluor® 647,
Excitation/Emission:Ex: 650nm, Em: 665nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Topoisomerase II alpha and Topoisomerase II beta also known as TOP2A and TOP2B are enzymes with a vital role in modulating the topological states of DNA. They actively introduce transient double-strand breaks in the DNA helix to manage DNA tangling and supercoiling during replication and transcription. TOP2A has a molecular weight of approximately 170 kilodaltons while TOP2B weighs around 180 kilodaltons. High levels of TOP2A are observed in proliferating cells such as those in the thymus and testis while TOP2B is expressed in nearly all tissues indicating its ubiquitous nature.
Biological function summary
These enzymes participate in fundamental cellular processes including chromosomal segregation replication and transcription. They function in the context of large protein complexes rather than in isolation. TOP2A is often engaged during mitosis due to its cell cycle-dependent expression. In contrast TOP2B's functions spread across various DNA-related actions such as differentiation and development. This distribution emphasizes their respective regulatory roles in both proliferating and resting cells.
Pathways
TOP2A and TOP2B integrate into critical pathways like the DNA replication and repair pathways. As a notable component of these pathways TOP2A interacts closely with proteins like MCM2-7 and ORC complex for replication origin unwinding while TOP2B shares interactions with factors like RNA polymerase II for transcription-related activities. These interactions reflect their involvement in maintaining genomic stability across different cellular conditions.
Aberrations in TOP2A expression or function link closely to numerous cancers reflecting its role in cell proliferation. Furthermore mutations or dysregulation of TOP2B have implications in disorders related to neural development highlighting its role beyond cellular growth control. Other related proteins like BRCA1 also display interactions with TOP2A reinforcing the enzyme's involvement in cancer-related pathways whereas TOP2B’s association with proteins like TAF(II)250 suggests a broader involvement in transcription processes relevant to neural disorders.