Abcam, ab201277, Mouse Eotaxin ELISA Kit (CCL11)

Size: 1 x 96Tests
Mouse Eotaxin ELISA Kit (CCL11) is a single-wash 90-min Simplestep used to quantify Mouse Eotaxin (CCL11) with a sensitivity of 8.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 8.1 pg/mL,
Range:23.44 - 1500 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Mouse Eotaxin ELISA Kit (CCL11) (ab201277) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Eotaxin (CCL11) protein in cell culture supernatant, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Eotaxin (CCL11) with 8.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Mouse Eotaxin (CCL11) is a 8.4 kDa cytokine, constitutively expressed in the thymus and inducibly expressed in the lung, intestine, heart, spleen and kidney. Eotaxin is a chemoattractant for eosinophils during inflammation and allergic reactions, and is also involved in mast cell differentiation and growth of myeloid cell progenitors.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Eotaxin also known as CCL11 or eotaxin-1 is a chemokine belonging to the CC chemokine family. This protein has a molecular mass of approximately 8.4 kDa. Eotaxin is mainly expressed in epithelial cells fibroblasts and smooth muscle cells. High levels of expression occur during inflammatory processes where it acts as a chemoattractant guiding eosinophils to sites of inflammation.
Biological function summary
Eotaxin plays an important role in immune response regulation particularly in allergic reactions. It functions by binding to CCR3 a receptor found on eosinophils therefore facilitating their migration. This process includes involvement in the formation of protein complexes that modulate cell signaling. Eotaxin's primary biological role lies in promoting the accumulation of eosinophils which are important for the immune defense against parasitic infections.
Pathways
Eotaxin participates in the MAPK/ERK signaling pathway which influences cell migration and proliferation. Additionally it operates within the NF-kB pathway often regulating inflammation and immune response. In these pathways eotaxin interacts with other proteins like CCR3 and IL-5 contributing to a coordinated response in immune signaling.
Eotaxin is closely linked to allergic asthma and atopic dermatitis. In both these conditions eotaxin-mediated eosinophil recruitment and activation worsen inflammation. Research shows significant eotaxin expression in asthmatic airway tissues. It also connects with proteins like IL-5 which amplify eosinophilic activity leading to exacerbated allergic responses.