Abcam, ab201279, Mouse E-Selectin ELISA Kit (CD62E)

Size: 1 x 96Tests
Mouse E-Selectin ELISA Kit (CD62E) is a single-wash 90-min Simplestep used to quantify Mouse E-Selectin (CD62E) with a sensitivity of 8.55 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Cell culture supernatant, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 8.55 pg/mL,
Range:23.4 - 1500 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Mouse E-Selectin ELISA Kit (CD62E) (ab201279) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of E-Selectin (CD62E) protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse E-Selectin (CD62E) with 8.55 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
E-Selectin is a cell-surface glycoprotein that plays a role in immune-adhesion. It is expressed by cytokine-stimulated endothelial cells, and mediates the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. E-selectin is also thought to have a role in capillary morphogenesis. Its structural features include the presence of lectin- and EGF-like domains followed by short consensus repeat domains that contain 6 conserved cysteine residues.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD62E also known as E-selectin or ELAM-1 is a cell adhesion molecule with a mass of approximately 115 kDa. It is present mainly on endothelial cells activated by cytokines. As a transmembrane glycoprotein E-selectin plays a mechanical role in mediating the tethering and rolling of leukocytes on the vascular endothelium during the inflammatory response. This function is important in directing leukocytes to sites of tissue damage or infection.
Biological function summary
E-selectin facilitates leukocyte adhesion by binding specific carbohydrate ligands on the surface of circulating immune cells. This binding is critical in the cascade of events that leads to leukocyte extravasation into tissues. E-selectin does not work in isolation rather forming part of a complex interaction with other cell adhesion molecules such as P-selectin and L-selectin. These interactions ensure precise control of cellular traffic during inflammatory responses.
Pathways
CD62E engages in the inflammatory signaling pathways including the NF-kB pathway. This pathway modulates the expression of E-selectin in response to pro-inflammatory cytokines like interleukin-1 and tumor necrosis factor-alpha. CD62E interacts with integrins on leukocytes and has downstream effects on cellular processes involved in immune response. Its cooperation with proteins like ICAM-1 and VCAM-1 further integrates it into a network of adhesion molecules maintaining vascular stability and immune surveillance.
E-selectin expression is highly relevant in inflammatory diseases such as rheumatoid arthritis and atherosclerosis. These disorders involve chronic inflammation where the persistent activation of endothelial cells and overexpression of E-selectin contribute to pathology. The link with ICAM-1 in these settings suggests a mutual regulation between cell adhesion molecules enhancing leukocyte recruitment to inflamed tissues. This makes E-selectin not only a marker of endothelial activation but also a potential therapeutic target for modulating leukocyte adhesion in inflammatory diseases.