Abcam, ab201280, Mouse Cystatin C ELISA Kit

Size: 1 x 96Tests
Mouse Cystatin C ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Cystatin C with a sensitivity of 5.25 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair - Cited in over 5 citations
Key facts
Detection method:Colorimetric,
Sample types:Urine, Tissue Extracts, Citrate plasma, Cell culture supernatant, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 5.25 pg/mL,
Range:23.4 - 1500 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Mouse Cystatin C ELISA Kit ab201280 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Cystatin C in cell culture supernatant, citrate plasma, serum, tissue extracts, urine. This SimpleStep sensitivity is 5.25 pg/mL.
How the assay works
Mouse Cystatin C SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse Cystatin C ELISA Kit ab201280 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Cystatin C antibody [EPR17639-19] - BSA and Azide free (Capture)
ab242743
- Anti-Cystatin C antibody [EPR17639-132] - BSA and Azide free (Detector)
ab242743
Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases. It is expressed in many organs of the body, and is an important extracellular inhibitor of cysteine proteases. As an inhibitor of cysteine proteinases, Cystatin C is thought to serve an important physiological role as a local regulator of this enzyme activity.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C