Abcam, ab201281, Rat Cystatin C ELISA Kit

Size: 1 x 96Tests
Rat Cystatin C ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Cystatin C with a sensitivity of 25.58 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair - Cited in over 5 citations
Key facts
Detection method:Colorimetric,
Sample types:Tissue Extracts, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Rat,
Assay type:Sandwich (quantitative),
Sensitivity:= 28.8 pg/mL,
Range:93.8 - 1500 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate

Product details:
Rat Cystatin C ELISA Kit (ab201281) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cystatin C protein in cell culture supernatant, cit plasma, edta plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Cystatin C with 25.58 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases. It is expressed in many organs of the body, and is an important extracellular inhibitor of cysteine proteases. As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C