Abcam, ab201282, Mouse IL-16 ELISA Kit

Size: 1 x 96Tests
Mouse IL-16 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-16 with a sensitivity of 3.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 3.6 pg/mL,
Range:10.93 - 700 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Mouse IL-16 ELISA Kit (ab201282) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-16 protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-16 with 3.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
IL-16 is a pro-inflammatory cytokine that stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. IL-16 primes CD4+ T-cells for IL-2 and IL-15 responsiveness. It also induces T-lymphocyte expression of interleukin 2 receptor. IL-16 is a ligand for CD4. IL-16 is synthesized in much larger 3 precursor isoforms localized in cytoplasm and nucleus and processed into 121 amino acid-long secreted IL-16. The isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane. The isoform 2 is involved in cell cycle progression in T-cells. Isoform 2 appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. It may act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C