Abcam, ab201366, Recombinant Flagellin protein

Size: 10µg
Recombinant Flagellin protein is a Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, HPLC.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag C-Terminus,
Applications:SDS-PAGE, HPLCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P06179,
Animal free:No,
Carrier free:No,
Species:Salmonella enterica subsp. enterica serovar Typhimurium str. LT2,
Reconstitution:Reconstitute in water or 0.1% BSA aqueous buffer,
Storage buffer:pH: 7.4Constituents: PBS

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Flagellin is a structural protein essential for the formation and function of bacterial flagella which is critical for motility. Also known as flagellin P41 this protein typically measures around 41 kDa in molecular mass. It plays an important role in the self-assembly of the flagellum's filament creating a helical structure. Expressed in motile bacterial species flagellin forms the building blocks that contribute to the rotation and movement of the flagellum allowing bacteria to navigate their environments.
Biological function summary
Flagellin proteins initiate immune responses as a pathogen-associated molecular pattern (PAMP). It forms part of the complex bacterial flagellar filament. When exposed to host organisms such as humans it can trigger innate immune responses via Toll-like receptor 5 (TLR5). This interaction stimulates cytokine production which can induce an immediate immune system reply to pathogenic bacterial invasion. Due to these interactions flagellin plays an essential role in bridging pathogen encounter and host defense mechanisms.
Pathways
Flagellin interacts significantly with the immune signaling system notably the NF-kB signaling pathway. Upon flagellin binding to TLR5 it activates a cascade leading to NF-kB pathway activation. This results in the transcription of various genes involved in inflammation and immune responses. Flagellin also links indirectly to the MAPK signaling pathway through TLR5 which mediates inflammatory responses. These pathways highlight flagellin's pivotal role in immune system activation and regulation.
Flagellin relates to inflammatory conditions such as inflammatory bowel disease (IBD) and sepsis. In IBD aberrant immune response to E. coli flagellin coupled with interactions with bacterial proteins can exacerbate intestinal inflammation. Sepsis a severe systemic inflammatory condition might involve excessive or uncontrolled response to flagellin leading to harmful immune activation. Understanding these relationships provides valuable insights into flagellin's role in pathological immune activation.