Abcam, ab201397, Recombinant human FGFR1OP-FGFR1 protein

Size: 5µg / 10µg
Recombinant human FGFR1OP-FGFR1 protein is a Human Fragment protein, in the 429 to 822 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>70% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:Tag free,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific activity of ab201397 determined by kinase assay was found to be 350 nmol/ min/ mg,
Accession:P11362,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.003% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% EDTA, 0.001% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The FGFR1OP-FGFR1 is a fusion protein resulting from the translocation between the FGFR1 oncogene and the FGFR1OP gene. This fusion is noteworthy in its mechanical role as it forms a constitutively active tyrosine kinase. The FGFR1OP-FGFR1 protein has a molecular mass that varies depending on the fusion breakpoint generally ranging from 100 to 120 kDa. Expressed predominantly in certain hematopoietic cells this fusion oncoprotein continually sends proliferative signals which alter normal cellular functions.
Biological function summary
FGFR1OP-FGFR1 fusion plays a significant role in driving abnormal cell growth and survival. The fusion protein is an active component within larger protein complexes that facilitate aberrant signaling. By bypassing typical regulatory mechanisms FGFR1OP-FGFR1 fusion protein leads to unchecked cellular division. In addition to maintaining survival pathways it disrupts normal differentiation processes in affected cells.
Pathways
FGFR1OP-FGFR1 is heavily implicated in the MAPK and PI3K/AKT signaling pathways which are central to cell cycle progression and survival. Key proteins related to these pathways include RAS RAF and AKT proteins which interact to propagate oncogenic signals downstream of the FGFR1OP-FGFR1 fusion. These interactions lead to heightened proliferative responses and resistance to apoptosis steering cells towards oncogenesis.
FGFR1OP-FGFR1 fusion is mostly connected to hematological malignancies mainly myeloproliferative neoplasms such as 8p11 myeloproliferative syndrome. Myelodysplastic syndromes have also shown associations with this protein. The FGFR1OP-FGFR1 fusion protein interacts closely with other oncoproteins like BCR-ABL enhancing its leukemogenic potential. Understanding and targeting this fusion protein is critical for effective therapies for these disorders.