Abcam, ab20193, Anti-HSV1 ICP8 Major DNA binding protein antibody [10A3]

Size: 50µg
Mouse Monoclonal DNBI antibody. Suitable for WB, ICC/IF and reacts with Herpes simplex virus, Purified native protein - Herpes simplex virus samples. Cited in 11 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:10A3,
Isotype:IgG1,
Light chain type:unknown,
Carrier free:No,
Reacts with:Herpes simplex virus,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A/G, Storage buffer-Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HSV1 ICP8 also known as the major DNA-binding protein is a vital component in the replication of herpes simplex virus type 1 (HSV1). Alternate names for this protein include infected cell protein 8 (ICP8) and 11E2. ICP8 boasts a molecular mass of approximately 128 kDa. This protein is expressed in HSV1-infected cells and exhibits strong DNA-binding properties. As a single-strand DNA-binding protein ICP8 facilitates the formation of complex viral replication machineries promoting helicase-primase activity necessary for viral DNA synthesis.
Biological function summary
ICP8 serves to orchestrate virus replication by acting at the core of the viral replication complex. It operates predominantly as a monomer but can form homo-oligomers in the presence of DNA. ICP8 interaction with other viral replication proteins such as UL5 UL52 and UL8 highlights its role as a scaffolding protein within the replication complex. Through binding and restructuring DNA ICP8 ensures efficient replication fork progression and genomic stability during HSV1 replication.
Pathways
ICP8 integrates critically into the replication pathway of HSV1. The replication process begins with initiation transitions through elongation and concludes with termination. Proteins related to these processes include UL9 which interacts with ICP8 to facilitate unwinding of the viral DNA. Another protein the polymerase-accessory protein UL42 works closely with ICP8 during the elongation of DNA synthesis. Through these interactions ICP8 plays a critical role in the orchestration and assembly of the HSV1 genome replication machinery.
ICP8 has a significant link to herpes simplex infections and diseases associated with viral latency and reactivation. HSV1 infections can lead to both oral herpes and more severe conditions such as herpes simplex encephalitis. The involvement of ICP8 extends to its interaction with cellular proteins that may affect immune evasion and viral persistence highlighting its potential importance in HSV1 pathogenesis. Understanding ICP8’s interaction with cellular proteins and its place in the disease cycle offers insight into therapeutic approaches targeting HSV1 infections.