Abcam, ab202762, Alexa Fluor® 488 Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9470]

Size: 100µL
Rabbit Recombinant Monoclonal eEF1A1/EF-Tu antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR9470,
Isotype:IgG,
Conjugation:Alexa Fluor® 488,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:EE1AL3/EEF1A1P5 it's a suspected pseudogene (https://www.ncbi.nlm.nih.gov/gene/158078). Our validation shows that this clone is able to detect Recombinant EF1AL3/EEF1A1P5 but endogenous protein expression is yet unknown.

Product details:
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The eEF1A1 also known as EF-Tu or eEF1A1-L3 is an elongation factor involved in protein synthesis. It facilitates the delivery of aminoacyl-tRNA to the A-site of the ribosome during translation. eEF1A1 exhibits a mass of approximately 50 kDa and is widely expressed in various tissues including the brain heart and skeletal muscle. Its expression level varies indicating that it plays a consistent role in translation machinery but may have tissue-specific functions.
Biological function summary
This elongation factor is important in promoting the accuracy and efficiency of protein translation. It interacts with several components of the protein synthesis machinery and forms part of a larger translational complex. eEF1A1 is involved in ensuring the correct amino acids are added during protein synthesis which affects protein structure and function. In addition to its canonical role it also engages in processes such as actin filament binding nuclear transport and other cell maintenance tasks.
Pathways
The activities of eEF1A1 intersect with key processes involved in cellular growth and proliferation. It plays a significant role in the mTOR signaling pathway which regulates cell growth by controlling protein synthesis. eEF1A1 also interacts with proteins like Rheb an important player in mTOR signaling. By participating in these pathways eEF1A1 links translation with broader regulatory networks that control cell metabolism.
EEF1A1 has connections to cancer and neurodegenerative diseases. Its elevated expression is often observed in several cancer types suggesting a role in tumorigenesis. Additionally misregulation of eEF1A1 may contribute to neurodegenerative disorders such as Alzheimer's disease where alterations in protein synthesis pathways are common. The protein's interaction with tau known in neuropathological conditions highlights its impact on neurodegeneration.