Abcam, ab202866, Anti-MCPyV_gp3 large T antigen antibody [Ab3]

Size: 100µg
Mouse Monoclonal MCPyV_gp3 large T antigen antibody. Suitable for WB, ICC/IF and reacts with Merkel cell polyomavirus samples. Cited in 5 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:Ab3,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Merkel cell polyomavirus,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:The epitope for monoclonal Ab3 is between amino acid residues 79 and 260 of MCPyV large T antigen. Ab3 does not bind to MCPyV small T antigen (PubMed ID: 23114601).

Product details:
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This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MCPyV_gp3 large T antigen also known as Merkel cell polyomavirus large T antigen is an important protein involved in the viral replication and transformation processes of Merkel cell polyomavirus (MCPyV). This antigen has an approximate mass of 90 kDa and is primarily expressed in cells infected by MCPyV. The large T antigen is named for its ability to bind to and inactivate tumor suppressor proteins which plays a role in the virus's oncogenic potential.
Biological function summary
The large T antigen serves as a multifunctional protein involved in viral replication cell cycle regulation and DNA damage response. Its function as a viral oncoprotein places it in the heart of tumor development processes. Large T antigen forms part of a complex with host cellular proteins assisting in the disruption of normal cellular controls that prevent tumor formation. This disruption is achieved by binding to cellular tumor suppressors such as p53 and retinoblastoma (Rb) proteins.
Pathways
The large T antigen is involved in cell cycle regulation and tumorigenesis pathways. It plays a substantial role in interfering with the Rb pathway which regulates cell cycle progression. This antigen also impacts the p53 pathway by binding and inactivating the p53 protein preventing apoptosis and contributing to uncontrolled cell proliferation. Both pathways are tightly regulated processes that when dysregulated contribute to oncogenesis.
The large T antigen of MCPyV is closely associated with Merkel cell carcinoma a rare but aggressive skin cancer. This cancer frequently arises from the integration of MCPyV DNA into the host genome and the expression of the large T antigen is a critical factor in these cancerous cells. Contacts with host proteins like p53 and Rb underline the antigen's role in the oncogenic processes leading to cancer development. Additionally research suggests potential associations with other disorders related to immune evasion given its role in manipulating host cellular machinery.