Abcam, ab202894, Anti-Aly/Ref antibody [EPR17942]

Size: 100µL / 1mL
Anti-Aly/Ref antibody [EPR17942] (ab202894) is a rabbit monoclonal antibody detecting Aly/Ref in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR17942,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IP, ICC/IF, IHC-P, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-Aly/Ref antibody [EPR17942] (ab202894) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of Aly/Ref?
Anti-Aly/Ref [EPR17942] (ab202894) specifically detects a band for Aly/Ref (UniProt: Q86V81) at a molecular weight of 27kDa.
Trusted by the scientific community
Anti-Aly/Ref [EPR17942] (ab202894) was first used in a scientific publication in 2015 and has been cited over 10 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
We have a range of other formats of antibody clone [EPR17942] also available for your convenience: ab202894, Carrier free -
ab240364
, PE -
ab305874
, APC -
ab305875
, HRP -
ab305876
, Alkaline Phosphatase -
ab308738
, Alexa Fluor® 488 -
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, Alexa Fluor® 647 -
ab310060
, Alexa Fluor® 594 -
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, Alexa Fluor® 555 -
ab311981
, Alexa Fluor® 568 -
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, Alexa Fluor® 750 -
ab321354
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Aly/Ref also known as THOC4 is a protein that plays an important role in mRNA processing and transport. It is a member of the TREX (transcription/export) complex having a molecular mass of approximately 27 kDa. Aly/Ref is primarily expressed in the nucleus where it binds to spliced mRNAs and ensures their export to the cytoplasm. Functionally Aly/Ref interacts with mRNA export adapters attaching to the cap-binding complex at the 5' end of mRNA facilitating its journey to the nuclear pore.
Biological function summary
Aly/Ref functions by forming tight associations with the TREX complex which includes other proteins like UAP56. It is responsible for coupling transcription with mRNA processing and export helping to maintain the integrity of gene expression. Aly/Ref directly influences the efficiency and accuracy of mRNA export impacting gene regulation by ensuring that only fully processed transcripts exit the nucleus. By doing so it helps coordinate the transcription and mRNA export processes.
Pathways
Aly/Ref associates with the mRNA surveillance pathway and the RNA export pathway. Aly/Ref works closely with proteins like EJC components which are involved in checking mRNA quality before export. This participation ensures that only correctly spliced mRNAs move from the nucleus to the cytoplasm where they translate into proteins. Aly/Ref also contributes to maintaining cellular homeostasis by regulating the amount of mRNA available for translation.
Aly/Ref relates to cancer and neurological disorders. Changes in the expression or function of Aly/Ref can disrupt mRNA export leading to abnormal protein synthesis which is associated with cancer development. Furthermore Aly/Ref interacts with proteins such as NXF1 which are implicated in the onset of certain neurological disorders when mutated or misregulated revealing its broader impact on cellular health and disease progression.