Abcam, ab203289, Anti-Acrosin antibody

Size: 100µL
Rabbit Polyclonal Acrosin antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human ACR aa 200-300 conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ACR aa 200-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P10323

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Proclin 300Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Acrosin also known as ZPC and with a mass of approximately 40 kDa acts as a proteolytic enzyme in sperm cells. It falls under the category of serine proteases. Acrosin is synthesized in the acrosome of spermatozoa and gets activated during the sperm's travel through the female reproductive tract. The enzyme's main mechanical function is to digest the zona pellucida the outer glycoprotein layer of the egg aiding in sperm penetration and fertilization process.
Biological function summary
Enzymes like acrosin play significant roles in fertilization. Acrosin remains stored as proacrosin its inactive zymogen form within the acrosome until it is activated. This protein operates as part of a complex network that includes other acrosomal proteins. The activation of acrosin is tightly controlled ensuring the precise timing and localization of its enzymatic activity required for efficient sperm-egg fusion.
Pathways
Sperm motility and capacitation pathways integrate acrosin activity. These pathways are pivotal during fertilization as they govern the sperm's ability to reach and penetrate the egg. Acrosin interacts with enzymes like hyaluronidase in pathways where the digestion of the zona pellucida is necessary. This interaction ensures that sperm cells effectively breach the barriers surrounding the oocyte for successful fertilization.
Fertilization problems can connect with acrosin function anomalies. Improper activation or deficits in acrosin activity may contribute to infertility. Additionally anomalies in related proteins such as other serine proteases or those within the sperm's acrosome can lead to unsuccessful sperm-egg interactions. Research focuses on exploring these relationships to identify potential diagnostic markers or therapeutic targets for addressing infertility issues associated with acrosomal proteins like acrosin.