Abcam, ab203323, Alexa Fluor® 647 Anti-MHC Class II beta antibody [EPR11227]

Size: 100µL
Rabbit Recombinant Monoclonal HLA-DPB1 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11227,
Isotype:IgG,
Conjugation:Alexa Fluor® 647,
Excitation/Emission:Ex: 650nm, Em: 665nm,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Stable for 12 months at -20°C, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The MHC Class II beta also known as HLA-DRB in humans is a protein essential for the immune system's functionality. It forms a part of the MHC class II protein complex with a typical mass around 28 kDa. Predominantly expressed on antigen-presenting cells such as dendritic cells B cells and macrophages it plays an important role in presenting extracellular antigens to CD4+ T helper cells. By doing so MHC Class II beta enables the adaptive immune response important for identifying and eliminating pathogens.
Biological function summary
MHC Class II beta facilitates the immune system's recognition of foreign particles. As part of the MHC class II complex it binds peptides derived from extracellular proteins processed in the endocytic pathway. This binding allows the presentation of peptide-MHC class II complexes on the cell surface which is recognized by CD4+ T cells thereby triggering T cell activation and proliferation. This mechanism is key for immune surveillance and for initiating the immune response against pathogens.
Pathways
The MHC Class II beta protein plays a significant role in the antigen processing and presentation pathways alongside proteins such as invariant chain (Ii) and HLA-DM. It is involved in the intracellular process where antigens are loaded onto MHC Class II molecules in the late endosome/lysosome. Additionally it is a part of the adaptive immune response pathway working in conjunction with other MHC proteins and immunoglobulins to mediate tolerance and immunity.
Defects or dysregulation in MHC Class II beta have been connected to autoimmune diseases such as rheumatoid arthritis and type 1 diabetes. Aberrant expression or function of MHC Class II molecules disturbs immune tolerance leading to self-antigen presentation and the breakdown of self-tolerance triggering autoimmune responses. The MHC Class II region is also associated with HLA-DRB alleles which have been implicated in increased susceptibility to these autoimmune conditions.