Abcam, ab203874, Anti-FILGAP antibody

Size: 100µL
Rabbit Polyclonal FILGAP antibody. Suitable for WB, IHC-P and reacts with Human, Rat samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human ARHGAP24 aa 600-700 conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ARHGAP24 aa 600-700 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.Q8N264

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Proclin 300Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FILGAP also known as ARHGAP24 is an important regulator in the Rho family of GTPases. This protein with a mass of approximately 67 kDa serves as a GTPase-activating protein (GAP) specific for Rac1. Through its action FILGAP inhibits Rac1-mediated signals which control actin dynamics. Expression of FILGAP occurs in various tissue types with significant levels in cells involved in cytoskeletal arrangement and cell movement such as fibroblasts and endothelial cells.
Biological function summary
FILGAP influences the rearrangement of actin cytoskeleton by inactivating Rac1 which is an essential factor for lamellipodia formation and cell migration. FILGAP interacts physically with the protein Filamin A suggesting its involvement in forming a protein complex that modulates actin cytoskeleton assembly. This interaction positions FILGAP at intricate cellular junctions where mechanical stimuli affect cell structure and signaling.
Pathways
The function of FILGAP fits largely within the Rho GTPase signaling pathway and the regulation of adherens junctions. It has interactions with other proteins including but not limited to Rac1 and Cdc42 linking it to pathways controlling cell migration and adhesion. Through its regulation of Rac1 FILGAP coordinates with pathways that affect cellular morphogenesis and mechanotransduction.
Aberrant expression or function of FILGAP links to cancer progression where its regulatory role impacts cell migration and invasion capabilities. Additionally disruptions in Rho GTPase pathways involving FILGAP contribute to cardiovascular disorders such as atherosclerosis due to its role in endothelial cell movement. In both contexts its interaction with Filamin A becomes significant influencing disease mechanisms tied to cytoskeletal abnormalities and cell motility.