Abcam, ab204517, Mouse MIP2 ELISA Kit (CXCL2)

Size: 1 x 96Tests
Mouse MIP2 ELISA Kit (CXCL2) is a single-wash 90-min Simplestep used to quantify Mouse MIP2 (CXCL2) with a sensitivity of 0.87 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair - Cited in over 5 citations
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.87 pg/mL,
Range:3.13 - 200 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse MIP2 ELISA Kit (CXCL2) ab204517 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse MIP2 (CXCL2)in cell culture supernatant, plasma, serum. This SimpleStep sensitivity is 0.87 pg/mL.
How the assay works
Mouse MIP2 (CXCL2)SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse MIP2 ELISA Kit (CXCL2) ab204517 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Mouse Macrophage Inflammatory Protein-2 (MIP2), also known as C-X-C motif chemokine 2 (CXCL2), is a small cytokine belonging to the CXC chemokine family. MIP2 was originally identified as a heparin-binding protein, and has been shown to exhibit potent neutrophil chemotactic activity. Mouse MIP2 c-DNA encodes a 100 amino acid residue precursor protein. The amino-terminal 27 amino acid residues are cleaved from this precursor to generate the mature mouse MIP2. Mouse MIP2 is 63% identical to Mouse KC (another mouse alpha chemokine), and mouse MIP2 is 60% identical to human GRO? and GRO?. Based on these protein sequence similarities, it is likely that mouse MIP2 and KC are homologs of the human GRO?, ?, and ? chemokines. However, since chemokines with protein sequence homology to human IL-8 have not been identified in mice, it has been suggested that the mouse MIP2 and KC are functional homologs of human IL-8 in mice. A putative mouse homolog of the human IL-8 receptor beta (IL-8 R?) has also been cloned. This receptor shows 71% identity to human IL-8 R? and 68% identity to human IL-8 R?. Both mouse MIP2 and KC bind mouse IL-8 R? with high affinity.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The CXCL2 protein also known as macrophage inflammatory protein 2 (MIP-2) or KC in the mouse model functions as a chemokine. It weighs approximately 7.8 kDa and is part of the CXC chemokine family. CXCL2 is mainly expressed in macrophages neutrophils and certain epithelial cells. It plays an important role in mediating the migration of these cells to sites of inflammation or injury.
Biological function summary
CXCL2 attracts and activates neutrophils as part of the innate immune response. It acts independently not as part of a larger complex to provoke a chemotactic response guiding neutrophils to areas of tissue injury. CXCL2 also promotes the release of other cytokines and enzymes that contribute to inflammation. Its activity is mediated through its interaction with receptors like CXCR2 on target cell surfaces.
Pathways
CXCL2 is involved in inflammatory signaling and leukocyte migration. It holds importance in the chemokine signaling pathway which regulates leukocyte trafficking. CXCL2 interacts with proteins like CXCR2 influencing the inflammation process by activating downstream kinases and other signal transduction molecules such as mitogen-activated protein kinases (MAPKs).
CXCL2 is connected to inflammatory conditions and diseases like sepsis and inflammatory bowel disease (IBD). Elevated levels of CXCL2 can enhance the inflammatory response which is linked to the progression of these conditions. The protein is also associated with tumor necrosis factor-alpha (TNF-alpha) which amplifies inflammation and tissue damage during disease processes.