Abcam, ab206314, Anti-Cdk1-2-3-5 antibody [EP762RY]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal CDK1 antibody. Suitable for IHC-P, WB and reacts with Human, Transfected cell lysate - Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP762RY,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cdk1 Cdk2 Cdk3 and Cdk5 also known as Cyclin-dependent kinases are essential regulators of the cell cycle. Cdk1 often called CDC2 has a molecular mass of approximately 34 kDa while Cdk2 Cdk3 and Cdk5 have similar masses. These proteins are serine/threonine kinases that become activated upon binding to specific cyclins with their expression most prominent in proliferating cells such as those in the brain and various tissues undergoing cell division. Cdk1 plays a role in G2-M transition in the cell cycle Cdk2 influences G1-S transition Cdk3 contributes to the entry into G1 and Cdk5 though not part of the traditional cell cycle is important in neuronal processes.
Biological function summary
Cyclin-dependent kinases drive essential cell cycle phases and are part of complex networks involving other cyclins. Cdk1 complexes with cyclin B to promote mitosis whereas Cdk2 typically interacts with cyclins A and E to facilitate DNA replication and S-phase entry. Cdk3 works less conventionally linking to transcriptional regulation mechanisms as well and Cdk5 often associated with p35 or p39 performs critical neuron-specific functions including synaptic signaling and neuronal development independently from the cell cycle.
Pathways
Cyclin-dependent kinases significantly impact the regulation of the cell cycle and apoptosis. They are vital in the p53 signaling pathway and cell cycle control pathway acting in coordination with retinoblastoma protein (Rb) and E2F transcription factors to regulate cell growth and repair. Cdk1 and Cdk2 share roles in checkpoint mechanisms for DNA damage ensuring genomic integrity before cell division. Cdk5 although not traditionally within cell cycle pathways influences pathways that affect neuroplasticity and neuron survival integrating with proteins such as tau and neurofilaments.
Cyclin-dependent kinases are critically linked to cancer and neurodegenerative diseases. In cancer their dysregulation especially Cdk1 and Cdk2 often leads to uncontrolled cell proliferation associated with cancer progression making them targets for anti-cancer therapies. In neurodegenerative conditions like Alzheimer's disease aberrant Cdk5 activity along with p25 formation can cause tau hyperphosphorylation contributing to neuronal dysfunction and death. Understanding these associations provides valuable insights into potential therapeutic strategies for managing these complex diseases.