Abcam, ab20664, Anti-Piccolo antibody

Size: 100µg
Rabbit Polyclonal Piccolo antibody. Suitable for IHC-P, WB, IHC (PFA fixed) and reacts with Rat, Mouse, Human samples. Cited in 13 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC (PFA fixed), IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Due to its large size, Piccolo requires special gel-electrophoresis and Western blotting protocols for visualization by immunoblotting. Excellent results can be obtained, for example, with the 4-12% TRIS-glycine gradient gels of Anamed. For success in WB with Rabbit polyclonal to Piccolo - Synaptic Marker (ab20664), do not denature WB sample lysate.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Piccolo also known as PCLO is a protein with a molecular weight of about 550 kDa. It is located at the presynaptic active zone of neurons where it performs essential mechanical roles. The presence of Piccolo is universal especially in the brain and it plays a critical part in maintaining the proper structure and function of synapses. The protein contributes to the assembly and modulation of the presynaptic scaffold aiding neurotransmitter release and synaptic vesicle cycling.
Biological function summary
Piccolo interacts with multiple components of the synaptic machinery as it is part of the active zone complex. It associates with proteins such as Bassoon which together help to modulate neurotransmission efficacy. Piccolo contributes to the regulation of synaptic vesicle pools shaping the speed and timing of synaptic responses. These interactions emphasize its role in synaptic plasticity and maintain overall neural network stability.
Pathways
Piccolo engages in the synaptic vesicle cycle and neurotransmitter release pathway. It connects closely with proteins such as Synapsin and Rab3A involved in these processes. Through these pathways Piccolo aligns presynaptic components to facilitate rapid synaptic responses. Consequently it plays a role in processes like learning and memory where fast neurotransmitter release is necessary.
Piccolo relates to neurological diseases like major depressive disorder and schizophrenia. Alterations in its expression or function impact synapse structure and may contribute to these conditions. It particularly intersects with proteins like Synapsin in the context of these disorders suggesting disruptions in neurotransmission can have wide-reaching effects on mental health and cognitive functions. These connections highlight the importance of Piccolo in maintaining normal neural function and its potential as a therapeutic target.