Abcam, ab206801, Recombinant human GSTM5 protein

Size: 20µg
Recombinant human GSTM5 protein is a Human Full Length protein, in the 1 to 218 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS, Mass Spec.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, FuncS, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific activity is > 90,000 pmol/min/μg, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25 degC.,
Accession:P46439,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GSTM5 also known as glutathione S-transferase Mu 5 is an enzyme that plays a pivotal role in detoxification processes. GSTM5 has a molecular mass of roughly 26 kDa. This protein belongs to the glutathione S-transferase family which are important for catalyzing the conjugation of glutathione to various substrates. GSTM5 is mainly expressed in tissues like the liver and lungs where detoxification is essential for maintaining cellular health.
Biological function summary
GSTM5 enzymes help in neutralizing toxins and products of oxidative stress by facilitating the conjugation of reduced glutathione to electrophilic compounds. GSTM5 does not function in isolation; it operates as part of the larger GST family that includes various isoforms like GSTM1 and GSTM3 sharing the responsibility of detoxifying harmful chemicals. Through its enzyme activity GSTM5 supports the protection of cells from toxic damage contributing to cellular antioxidant defense.
Pathways
GSTM5 activity contributes to the metabolic pathway involving the detoxification of xenobiotics playing a role in phase II metabolism. It is strongly connected to the glutathione conjugation pathway helping in the clearance of lipid peroxidation products. GSTM5 interacts closely with other proteins in the GST family including GSTP1 pinpointing its role in maintaining cellular redox balance and aiding in the conversion of harmful substances into more water-soluble products for excretion.
GSTM5 is associated with cancer and neurodegenerative diseases. Variations in GSTM5 expression have been linked to increased risk in the development of certain cancers given its role in detoxifying carcinogens. Moreover GSTM5 may influence the progression of neurodegenerative disorders by modulating oxidative stress responses a process where its counterpart GSTP1 also plays a significant part. Understanding GSTM5’s function can offer insights into potential therapeutic approaches for these conditions.