Abcam, ab207248, Alexa Fluor® 405 Anti-NEDD8 antibody [Y297]

Size: 100µL
Rabbit Recombinant Monoclonal NEDD8 antibody - conjugated to Alexa Fluor® 405. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:Y297,
Isotype:IgG,
Conjugation:Alexa Fluor® 405,
Excitation/Emission:Ex: 401nm, Em: 421nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NEDD8 also known as neural precursor cell expressed developmentally downregulated protein 8 is a small ubiquitin-like protein with a molecular weight of approximately 9 kDa. It is expressed in various tissues prominently in the brain and muscles. NEDD8 conjugates to target proteins modifying their functions by altering their stability activity or location. It plays a role in the regulation of protein degradation.
Biological function summary
The NEDD8 protein is part of a modification system that activates cullin-RING ligase complexes. This activation promotes the ubiquitination and subsequent proteasomal degradation of targeted proteins. As a post-translational modification system similar to ubiquitin NEDD8 is important for maintaining protein homeostasis by ensuring timely degradation and turnover of cellular proteins. These processes impact cell cycle progression signal transduction and stress responses.
Pathways
NEDD8 is a central player in the ubiquitin-proteasome system an essential pathway for protein turnover. It is particularly important in the NEDDylation pathway where it regulates protein functions by transiently attaching to substrate proteins. Through these processes NEDD8 interacts closely with proteins like cullins and ubiquitin affecting cellular responses and maintaining cellular integrity under different conditions.
NEDD8 influences several pathological states. Aberrant NEDD8 conjugation can lead to cancer by disrupting the controlled degradation of proteins involved in cell cycle regulation such as p27 and p21. Additionally improper NEDDylation has links to neurodegenerative diseases like Alzheimer's as it affects proteins related to synaptic function and neuronal survival. Understanding the role of NEDD8 in these diseases aids in the development of potential therapeutic strategies.