Abcam, ab20737, Anti-Apolipoprotein B antibody

Size: 500µL
Anti-Apolipoprotein B antibody (ab20737) is a rabbit polyclonal antibody detecting Apolipoprotein B in Western Blot . Suitable for Human, Mouse . - Over 80 publications - Trusted since 2005
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Mouse Apob.E9Q414,
Specificity:Cross-reacts with Apo B48 and B100.

Product details:
What is this antibody validated in?
Anti-Apolipoprotein B antibody (ab20737) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse samples.
What is the molecular weight of Apolipoprotein B?
Anti-Apolipoprotein B (ab20737) specifically detects a band for Apolipoprotein B (UniProt: P04114) at a molecular weight of 515kDa.
Trusted by the scientific community
Anti-Apolipoprotein B (ab20737) was first used in a scientific publication in 2005 and has been cited over 80 times in peer-reviewed journals.
Reviewed by scientists
Anti-Apolipoprotein B (ab20737) has over 5 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Purification notes-Heat inactivated.Product is 0.22um filtered., Storage buffer-Preservative: 0.02% Sodium azideConstituents: Whole serum, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Apolipoprotein B often referred to as ApoB is a core protein component of low-density lipoproteins (LDL) and is essential for their assembly and secretion. ApoB is also known as apolipoproteine B and apolipoprotein B protein. This protein has a large molecular weight approximately 550 kDa. ApoB is primarily expressed in the liver and to some extent in the intestine. Its large size allows it to provide structural integrity to lipoprotein particles playing a role in lipid metabolism.
Biological function summary
ApoB plays a significant role in lipid transport and metabolism. It is a critical component of the lipoprotein particle complex which is responsible for the transport of lipids such as cholesterol and triglycerides through the bloodstream. ApoB helps in the binding of lipoproteins to cell receptors facilitating the uptake of lipids by tissues. Its presence on the surface of lipoproteins makes it important for normal cholesterol homeostasis in the human body.
Pathways
ApoB is integrated within the lipid metabolism pathway. It interacts with proteins such as microsomal triglyceride transfer protein (MTP) during the assembly and secretion of lipoproteins. This pathway is essential for the normal transport of lipids throughout the body and influences processes like absorption of dietary fats. ApoB also plays a role in the pathways related to atherosclerosis where it influences the deposition of cholesterol in arterial walls.
ApoB is well-associated with cardiovascular diseases notably atherosclerosis and familial hypercholesterolemia. In these conditions elevated levels of ApoB correlate to an increased risk of plaque formation in blood vessels. ApoB in conjunction with LDL receptor and other related proteins influences lipid homeostasis and disorders where lipid oxidation or accumulation is present. Monitoring ApoB and its concentration can be vital for assessing cardiovascular disease risk.