Abcam, ab207621, Human Heme Oxygenase 1 ELISA Kit

Size: 1 x 96Tests
Human Heme Oxygenase 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Heme Oxygenase 1 with a sensitivity of 7.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair - Cited in over 15 citations
Key facts
Detection method:Colorimetric,
Sample types:Cell culture extracts, Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Tissue Extracts,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 7.9 pg/mL,
Range:23.44 - 1500 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Human Heme Oxygenase 1 ELISA Kit ab207621 is a rapid single-wash 90-min Sandwich ELISA to measure Human Heme Oxygenase 1 in cell culture extracts, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 7.9 pg/mL.
How the assay works
Human Heme Oxygenase 1 SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Heme Oxygenase 1 ELISA Kit ab207621 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Heme Oxygenase 1 antibody [EPR18689-5] - BSA and Azide free (Capture)
ab242982
- Anti-Heme Oxygenase 1 antibody [EPR18689-126] - BSA and Azide free (Detector)
ab242982
Heme oxygenase 1 (HO 1) is an enzyme that functions in heme catabolism. The activity of heme oxygenase 1 is to cleave the heme ring to form biliverdin. There are two isozymes of heme oxygenase that have 56% amino acid sequence identity: inducible heme oxygenase 1 and constitutively expressed heme oxygenase 2.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Heme oxygenase 1 (HO-1) also known as heme oxygenase-1 or oxygenase catalyzes the degradation of heme into biliverdin carbon monoxide and free iron. It has been characterized with a mass of approximately 32 kDa. HO-1 is widely expressed in tissues with high metabolic activity such as liver spleen bone marrow and brain. This enzyme plays key roles in cellular response to oxidative stress and is responsive to various stress-inducing stimuli.
Biological function summary
The function of HO-1 involves important antioxidant defense mechanisms. The enzyme contributes to the cytoprotective effects by moderating oxidative stress and inflammation through its enzymatic activity. HO-1 often forms part of a larger regulatory complex where its activity regulates other cellular processes. Its heme byproducts biliverdin and bilirubin possess strong antioxidant properties adding to the cell's capacity to neutralize harmful oxidative effects.
Pathways
HO-1 operates in heme degradation and the antioxidative response pathway. It closely interacts with ferritin in its role of managing intracellular iron levels. As HO-1 breaks down heme the free iron released is sequestered by ferritin preventing cellular iron overload. HO-1 also plays a part in the signaling pathway involved in maintaining vascular homeostasis where it may modulate the activity of nitric oxide synthase through its byproducts.
Dysregulation of HO-1 levels connects to conditions such as cardiovascular diseases and certain neurodegenerative disorders. In cardiovascular diseases especially atherosclerosis the improper balance of heme oxygenase-1 activity can influence the severity of oxidative damage. Furthermore HO-1 relates to the pathogenesis of Alzheimer's disease where oxidative stress plays a significant role and where its interaction with proteins like amyloid-beta if disrupted may worsen disease pathology.