Abcam, ab208199, Anti-1-methylguanosine (m1G) antibody [EPR19833-150]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal 1-methylguanosine (m1G) antibody. Suitable for IP, ELISA, FRET, Dot and reacts with Chemical, Synthetic peptide samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19833-150,
Isotype:IgG,
Carrier free:No,
Applications:Dot, FRET, IP, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
1-methylguanosine (m1G) is a modified nucleoside with a distinct function in RNA biology. It is a methylated derivative of guanosine. This target plays an important role in RNA modification and stability. m1G results from the methylation of guanosine at the first position and has a molecular mass of about 167.15 g/mol. Expression of m1G occurs in various types of RNA across eukaryotic and prokaryotic organisms showing its widespread significance in biological systems. Although m1G itself is not a protein its presence impacts the mechanical aspects of the RNA molecule where it resides.
Biological function summary
M1G impacts RNA functionality by influencing RNA folding and structural conformation. It forms integral parts of RNA molecules and contributes to their stability and function. m1G does not act in isolation but is part of a broader set of modified nucleosides that collectively enhance RNA function. The modifications involving m1G occur as part of post-transcriptional modification processes. These are often important for ensuring proper decoding and function of RNA during gene expression.
Pathways
Research indicates that the presence of m1G within RNA molecules can influence specific biological pathways related to translation and RNA stability. m1G impacts the efficiency and fidelity of translational machinery playing a part in the translation pathway. Additionally it interacts with various proteins like tRNA methyltransferases which facilitate its methylation in RNA. These enzymes are critical in pathways that ensure the correctness of RNA modifications which ultimately influence protein synthesis.
Researchers associate aberrant levels of m1G with certain conditions such as neurodegenerative diseases and cancer. Disruption in the normal modification process of guanosine to m1G can lead to dysfunctional RNA which relates to disease pathology. Disorders in m1G modification often implicate other proteins involved in RNA modification like small nucleolar RNAs (snoRNAs) which assist in inserting this modification. Understanding m1G's role in disease progression helps develop targeted therapeutic strategies focusing on the modulation of these RNA-modifying enzymes.