Abcam, ab208348, Mouse TNF alpha ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests
Mouse TNF alpha ELISA kit is a single-wash, 90-minute SimpleStep ELISA® designed to quantify mouse TNF alpha with a sensitivity of 9.1 pg/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric sandwich ELISA -450 nm readout; works on any plate reader - Different formats for different needs – 384-well format for higher throughput - Broad sample compatibility – works with serum, plasma (heparin, citrate, EDTA), and cell culture supernatants - Cited in over 235 publications
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 9.1 pg/mL,
Range:46.88 - 3000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse TNF alpha ELISA Kit ab208348 is a rapid single-wash 90-min ELISA kit to measure cell culture supernatant, Serum, plasma. This SimpleStep ELISA® sensitivity is 9.1 pg/mL.
How the assay works
SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant mouse TNF alpha protein in serum, plasma, and cell culture supernatant samples only.
Recombinant mouse TNF RI, TNF RII, OPG, CD40 receptor, IL1 beta, IL-16, and IL-5 were prepared at 100 ng/mL and assayed for cross reactivity. No cross-reactivity was observed. Recombinant human TNF alpha was prepared at 100 ng/mL, 25 ng/mL, and 3 ng/mL and assayed for cross reactivity. On average, 3.0% cross-reactivity was observed with a standard deviation of 0.7%.
Recombinant mouse TNF RI, TNF RII, OPG, CD40 receptor, IL1 beta, IL-16, and IL-5 and recombinant human TNF alpha were prepared at 100 ng/mL and tested for interference. No interference with was observed.
This immunoassay is calibrated against a highly purified mouse TNF alpha. The NIBSC/WHO unclassified purified mouse TNF alpha preparation 88/532 was evaluated in this kit. The dose response curve of the unclassified standard 88/532 parallels the SimpleStep standard curve. To convert sample values obtained with the SimpleStep mouse TNF alpha kit to approximate NIBSC 88/532 units, use the equation below.
NIBSC (88/532) approximate value (units/mL) = 2.0 x SimpleStep mouse TNF alpha value (pg/mL)
Mouse TNF alpha ELISA Kit ab208348 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Mouse TNF alpha ELISA Kit ab208348
Mouse TNF alpha ELISA Kit ab208348 has been used to assess inflammation in different physio-pathological contexts such as liver disease
, aging
, and Alzheimer's disease
References:
Ai-Lei Xu et al. 2023, PMID: 37902450,
Yang Bao et al. 2023, PMID: 37954634,
Ramon Velazquez et al. 2023, PMID: 37548694
Save your precious samples
Our 384-well Mouse TNF alpha ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Mouse TNF alpha Antibody Pair - BSA and Azide free
ab241672
- Anti-TNF alpha antibody [EPR16803-2] - BSA and Azide free (Capture)
ab242949
- Anti-TNF alpha antibody [EPR16803-84] - BSA and Azide free (Detector)
ab242977
Related and recommended products
Mouse TNF alpha ELISA kit ab208348 is commonly used to measure TNF alpha as a marker of inflammation.
Other ELISA kits to measure inflammatory markers include:
- Mouse IL-6 ELISA kit
ab222503
- Mouse IFN gamma ELISA kit
ab282874
- Mouse IL-1 beta ELISA kit
ab197742
Tumor necrosis factor, or TNF alpha, is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. Furthermore, TNF alpha is a ligand of the TNF superfamily which plays a central role in inflammation, apoptosis, proliferation, invasion, angiogenesis, metastasis, and morphogenesis. It is mainly secreted by macrophages, and can induce cell death of certain tumor cell lines. Rat and Human TNF alpha are 95% and 79% identical to Mouse TNF alpha, respectively. TNF alpha is expressed on macrophages and endothelial, epithelial, and tumor cells as a 26 kDa transmembrane protein. TNF-alpha is cleaved by proteolytic processing into six chains: (1) TNF membrane form, (2) Intracellular domain 1 (ICD1), (3) Intracellular domain 2 (ICD2), (4) C-domain 1, (5) C-domain 2 and (6) TNF soluble form. ICD1 and ICD2 are released into the cytosol, while C-domain 1 and C-domain 2 are released into the extracellular space. Furthermore, the membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. Signaling from TNF-alpha differs depending on the type of ligand initiating the signaling event (intracellular, membrane, or soluble). As an example, the membrane form of TNF-alpha appears to mediate anti-tumorigenic therapeutic responses whereas the soluble ligand is linked to inflammation and proliferation. Likewise, the TNF intracellular domain (ICD) form induces IL-12 production in dendritic cells.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C