Abcam, ab208774, Anti-Pro Caspase-8 antibody [EPR162] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Caspase-8 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR162,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Theoretically, this antibody should recognize p55/54, p43/41 and p18. However, in our internal tests on parent antibody ab108333, it only recognized pro caspase 8. We tested it side by side with ab32397. Both antibodies are KO validated, and ab32397 recognizes pro-caspase 8 and cleavage caspase 8.

Product details:
ab208774 is the carrier-free version of
ab108333
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pro Caspase-8 also known as CASP8 is an inactive precursor of caspase-8 an important enzyme in the apoptosis and necroptosis pathways. This protein has a molecular mass of approximately 55 kDa. Caspase-8 expression occurs in various tissues with higher prevalence in immune cells including T lymphocytes and natural killer cells. Researchers often use techniques like western blot for caspase 8 detection particularly when analyzing its activation state.
Biological function summary
Pro caspase-8 plays a significant role in the regulation of apoptosis a programmed cell death process. It is initially synthesized in an inactive form and requires activation by cleavage to become functional. Pro caspase-8 integrates into the death-inducing signaling complex (DISC) following specific triggers such as Fas receptor engagement or TNF receptor activation indicating its role in response to external apoptotic signals. Its activation marks the initiation of a cascade that results in apoptosis ensuring controlled cell removal in multicellular organisms.
Pathways
Pro caspase-8 functions within the extrinsic apoptosis pathway where it serves as an initiator caspase. Alongside other proteins like Fas-associated protein with death domain (FADD) caspase-8 forms a critical part of the apoptotic signaling network. Another important pathway involving pro caspase-8 is necroptosis an alternative form of programmed cell death where it can inhibit the function of receptor-interacting protein kinase 1 (RIPK1) and RIPK3 under certain conditions. The regulation of these pathways highlights pro caspase-8's central role in determining cell fate under stress or damage.
Aberrations in pro caspase-8 activity link to various conditions such as cancer and autoimmune diseases. In cancers insufficient caspase-8 activation results in the evasion of apoptosis allowing uncontrolled cell proliferation. Meanwhile excessive or improper activation associates with autoimmune disorders where unregulated apoptosis leads to immune system imbalance. Pro caspase-8 connects with proteins like FLICE-like inhibitory protein (c-FLIP) in cancer where it influences the apoptotic threshold and contributes to the pathology.