Abcam, ab208943, Anti-LAMP1 antibody [EPR21026] - Lysosome Marker

Size: 100µL / 1mL
Anti-LAMP1 antibody [EPR21026] (ab208943) is a rabbit monoclonal antibody detecting LAMP1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, IHC-Fr, ICC/IF . Suitable for Mouse . - Biophysical QC for unrivalled batch-batch consistency - Over 30 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR21026,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:Flow Cyt (Intra), IHC-Fr, IHC-P, WB, ICC/IF, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:In our lab we observe staining in multiple tissues (spleen, lung, kidney etc.) in IHC-P with ab208943, but a lack of staining on mouse brain. We have received feedback from other researchers, that they also do not see staining in mouse brain with this antibody. Therefore we do not recommend using this reagent, for work on mouse brain, in IHC-P. For further information on this please contact our Technical Support team who will be happy to help.

Product details:
Anti-LAMP1 antibody [EPR21026] (ab208943) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, IP, WB in mouse samples.
Anti-LAMP1 antibody [EPR21026] (ab208943) has been cited over 31 times in peer reviewed journals and is trusted by the scientific community.
Abcams high quality manufacturing and validation processes ensure Anti-LAMP1 antibody [EPR21026] (ab208943) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-LAMP1 antibody [EPR21026] (ab208943) specifically detects LAMP1 (UniProt ID: P11438; Molecular weight: 42kDa) and is sold in 100 uL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EPR21026 -
ab225762
Antibody clone EPR21026 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, Alexa Fluor® 555, Alexa Fluor® 594, Alexa Fluor® 568, PE (ab23737, ab355, ab32684,
ab3282
ab314256
Lysosomal-associated membrane protein 1 (LAMP1) is an essential lysosomal membrane protein that plays a crucial role in cellular processes such as waste degradation and autophagy regulation. It is a significant focus of research in neuroscience and cell biology due to its importance in maintaining cellular health. Changes in LAMP1 expression are associated with various diseases, including cancer, neurodegenerative disorders like Alzheimer's disease (AD), and lysosomal storage diseases. Studying LAMP1 provides valuable insights into the mechanisms of these diseases and the cellular dysfunctions involved, highlighting its critical role in understanding disease pathology.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb
patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LAMP1 also known as lysosome-associated membrane glycoprotein 1 is an important player within cellular mechanics. It exhibits a molecular weight of approximately 120 kDa. This protein exists abundantly in lysosomal membranes and sometimes in endosomes. LAMP1 operates near most cell types and has a significant presence in immune neuronal and epithelial cells. By maintaining the lysosomal membrane's integrity LAMP1 contributes to cellular homeostasis.
Biological function summary
LAMP1 functions to protect lysosomal membranes from the harsh environment inside the lysosome. It forms part of a protective glycocalyx composed of highly glycosylated proteins supporting lysosomal stability. This protein interacts closely with other lysosome markers and assists in the fusion of vesicles with lysosomes making it fundamental to lysosomal processes. As a lysosome marker LAMP1 helps identify lysosomal compartments during studies involving microscopy and LAMP1 staining.
Pathways
The protein LAMP1 participates in the autophagy and endocytic pathways. It acts collaboratively within the lysosomal degradation route involving proteins like LAMP2 which also supports lysosomal function. LAMP1 influences these pathways by mediating the fusion of autophagosomes or endosomes with lysosomes thereby ensuring efficient breakdown of cellular debris and macromolecules.
LAMP1 associates with lysosomal storage disorders and neurodegenerative diseases. The abnormal function or expression of LAMP1 has been linked to conditions such as Niemann-Pick disease and Alzheimer's disease. In these contexts its interaction with proteins like APP (amyloid precursor protein) illustrates its involvement in pathogenic pathways that lead to cellular dysfunction and disease progression. Understanding LAMP1's role may enhance diagnostic and therapeutic approaches pertaining to these disorders.