Abcam, ab208985, Mouse CCL21a ELISA Kit

Size: 1 x 96Tests
Mouse CCL21a ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CCL21a with a sensitivity of 1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 1 pg/mL,
Range:3.91 - 250 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Mouse CCL21a ELISA Kit (ab208985) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL21a protein in cell culture extracts, cell culture supernatant, serum, tissue extracts, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CCL21a with 1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Mouse CCL21a is a member of the CC subfamily of chemokines. Chemokines are a class of cytokines that induce chemotaxis of target cells. CCL21a is highly expressed in the lymph nodes and chemoattracts naïve T cells and mature dendritic cells into T zones of secondary lymphoid organs. Furthermore, it has been suggested that CCL21a induces human microvascular endothelial cell (HMVEC) migration and/or tube formation. In addition, it has been reported that sustained high concentrations of soluble or immobilized CCL21a inhibits T cell proliferation and IL-2 secretion. Human CCL21a exhibits 71% amino acid identity to mouse CCL21a.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C-C motif chemokine ligand 21a also known as Ccl21a or Exodus-2 is a chemokine with a molecular mass of approximately 16 kDa. It functions mechanically by acting as a ligand for the CCR7 receptor which plays a significant role in immune responses. Ccl21a is mainly expressed in lymphoid tissues such as the lymph nodes and the spleen where it guides naive T-cells and dendritic cells to these regions by creating a chemotactic gradient.
Biological function summary
The actions of Ccl21a are important for the immune system's homeostasis and surveillance. It helps maintain the organized structure of lymphoid tissues by ensuring the correct positioning of lymphocytes. Ccl21a operates as part of the network that forms the microenvironment necessary for immune cell interactions and antigen presentation. There are no notable reports of it forming part of a larger protein complex but its receptor CCR7 is an essential player in its function.
Pathways
Ccl21a is involved in the chemokine signaling pathway and the regulation of immune cell trafficking. It interacts heavily within the pathway that involves the engagement of CCR7 a receptor that Ccl21a primarily activates alongside its counterpart ligand CCL19. Through these interactions Ccl21a influences processes related to lymphocyte migration and adaptive immune responses.
Disruptions in Ccl21a expression or function associate with conditions like autoimmune diseases and chronic inflammation. Abnormalities in its signaling can result in diseases such as rheumatoid arthritis due to inappropriate recruitment and positioning of immune cells. Ccl21a's interaction with proteins like CCR7 links it to these disorders as aberrant CCR7 engagement influences immune cell dynamics in diseased states.